Detailed information of TA system TA175631

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2644526..2644751
• Replicon: chromosome
• Accession: NZ_CP062717
• Organism: Escherichia coli O157:H7 strain Z1825

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ56_RS13415
• Protein ID: WP_000813258.1
• Coordinates: 2644596..2644751 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2644526..2644584 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ56_RS13365	2639591..2639833	+	243	WP_000747948.1	hypothetical protein	-
ILQ56_RS13370	2639817..2640242	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ56_RS13375	2640311..2641354	+	1044	WP_001262402.1	hypothetical protein	-
ILQ56_RS13380	2641347..2641808	+	462	WP_000139447.1	replication protein	-
ILQ56_RS13385	2641842..2642558	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ56_RS13390	2642591..2642872	+	282	WP_000603384.1	DNA-binding protein	-
ILQ56_RS13395	2642869..2643096	+	228	WP_000699809.1	hypothetical protein	-
ILQ56_RS13400	2643089..2643400	+	312	WP_001289673.1	hypothetical protein	-
ILQ56_RS13405	2643528..2643746	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ56_RS13410	2643748..2644305	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2644526..2644584	-	59	-	-	Antitoxin
ILQ56_RS13415	2644596..2644751	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ56_RS13420	2644871..2645215	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ56_RS13425	2645337..2645609	+	273	WP_000191872.1	hypothetical protein	-
ILQ56_RS13430	2645611..2646660	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ56_RS13435	2646673..2646978	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ56_RS13440	2647041..2647595	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ56_RS13445	2647820..2648017	+	198	WP_000917763.1	hypothetical protein	-
ILQ56_RS13450	2648153..2648866	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ56_RS13465	2649317..2649748	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2575132..2729455	154323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175631 WP_000813258.1 NZ_CP062717:2644596-2644751 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175631 NZ_CP082120:1857390-1857641 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175631 NZ_CP062717:c2644584-2644526 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin