Detailed information of TA system TA175595

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2622103..2622328
• Replicon: chromosome
• Accession: NZ_CP062715
• Organism: Escherichia coli O157:H7 strain Z1826

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ILQ57_RS13240
• Protein ID: WP_000813258.1
• Coordinates: 2622173..2622328 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2622103..2622161 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ57_RS13190	2617168..2617410	+	243	WP_000747948.1	hypothetical protein	-
ILQ57_RS13195	2617394..2617819	+	426	WP_000693878.1	Rha family transcriptional regulator	-
ILQ57_RS13200	2617888..2618931	+	1044	WP_001262402.1	hypothetical protein	-
ILQ57_RS13205	2618924..2619385	+	462	WP_000139447.1	replication protein	-
ILQ57_RS13210	2619419..2620135	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
ILQ57_RS13215	2620168..2620449	+	282	WP_000603384.1	DNA-binding protein	-
ILQ57_RS13220	2620446..2620673	+	228	WP_000699809.1	hypothetical protein	-
ILQ57_RS13225	2620666..2620977	+	312	WP_001289673.1	hypothetical protein	-
ILQ57_RS13230	2621105..2621323	+	219	WP_000683609.1	DUF4014 family protein	-
ILQ57_RS13235	2621325..2621882	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2622103..2622161	-	59	-	-	Antitoxin
ILQ57_RS13240	2622173..2622328	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
ILQ57_RS13245	2622448..2622792	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ILQ57_RS13250	2622914..2623186	+	273	WP_000191872.1	hypothetical protein	-
ILQ57_RS13255	2623188..2624237	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
ILQ57_RS13260	2624250..2624555	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ILQ57_RS13265	2624618..2625172	+	555	WP_000640035.1	DUF1133 family protein	-
ILQ57_RS13270	2625397..2625594	+	198	WP_000917763.1	hypothetical protein	-
ILQ57_RS13275	2625730..2626443	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ILQ57_RS13290	2626894..2627325	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleG7' / nleC	2561742..2705244	143502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T175595 WP_000813258.1 NZ_CP062715:2622173-2622328 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T175595 NZ_CP082037:328668-329036 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT175595 NZ_CP062715:c2622161-2622103 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin