Detailed information of TA system TA175422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4646967..4647517
• Replicon: chromosome
• Accession: NZ_CP062702
• Organism: Escherichia coli O157:H7 strain Z1834

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: ILQ62_RS23110
• Protein ID: WP_001160975.1
• Coordinates: 4646967..4647281 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: ILQ62_RS23115
• Protein ID: WP_000125566.1
• Coordinates: 4647284..4647517 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ62_RS23085	4642642..4643928	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
ILQ62_RS23090	4643928..4644917	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
ILQ62_RS23095	4644914..4645735	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
ILQ62_RS23100	4645738..4646115	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
ILQ62_RS23105	4646122..4646970	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
ILQ62_RS23110	4646967..4647281	-	315	WP_001160975.1	CcdB family protein	Toxin
ILQ62_RS23115	4647284..4647517	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
ILQ62_RS23120	4647603..4648082	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
ILQ62_RS23125	4648274..4650136	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
ILQ62_RS23130	4650129..4650659	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
ILQ62_RS23135	4650767..4652098	-	1332	WP_001183177.1	MFS transporter	-
ILQ62_RS23140	4652156..4652443	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T175422 WP_001160975.1 NZ_CP062702:c4647281-4646967 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T175422 NZ_CP081939:1469193-1469375 [Avibacterium paragallinarum]
ATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGTAAAGTCGAAAATACAAAGCGAGGAAGCCATCGCAA
GGTAACGTTGAACGGACACCAAACTGTATTTCCCTACCACGGTAGTCAAGAAATCGGAACAGGGTTAGTTCACAAAATCA
AAAAAGACTTAAATTTAAAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT175422 WP_000125566.1 NZ_CP062702:c4647517-4647284 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT175422 NZ_CP081939:1469428-1469847 [Avibacterium paragallinarum]
ATGTTACGTTATCCTGTTGAATTAAAAGCCGATGATAACGGCACGTTTCTTGTTACCTTTCCTGATATTCCCGAAGCGGC
AAGTGTAGGAGAAGACCTTGAAAGTGCCTTACTTGAAGCAGAGGAAGGATTAGAAACCGCACTGGAATTTTATTTTGATG
ATAAACGCCCAATCCCATTACCGAGTAAACCTCAAGAGGGGCAATATACTGTTCCGCTCTCTTTATTGCGCTCATTAAAA
GTGTTATTACTGAATGAAATGCTCGCTCAAGGTGTTCGCAAAGCAGAAATGGCTCGTCGCCTTGATGTACATATGCCACA
AATCGATCGCTTATTAGATTTTCGCTATCCTTCTAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAACTAGGGCGGGAAA
TTCATTTGGCGGTGAGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9