Detailed information of TA system TA175158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1613429..1613958
• Replicon: chromosome
• Accession: NZ_CP062416
• Organism: Staphylococcus aureus strain NAS_AN_149

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX2
• Locus tag: IF719_RS08010
• Protein ID: WP_001103933.1
• Coordinates: 1613429..1613746 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: A0A2X2M557
• Locus tag: IF719_RS08015
• Protein ID: WP_001058488.1
• Coordinates: 1613749..1613958 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF719_RS07985 (1608544)	1608544..1608885	-	342	WP_001190614.1	hypothetical protein	-
IF719_RS07990 (1609421)	1609421..1610062	-	642	WP_064140196.1	pathogenicity island protein	-
IF719_RS07995 (1610059)	1610059..1610439	-	381	WP_000356927.1	hypothetical protein	-
IF719_RS08000 (1610770)	1610770..1612479	-	1710	WP_000447454.1	DUF927 domain-containing protein	-
IF719_RS08005 (1612493)	1612493..1613362	-	870	WP_001002701.1	primase alpha helix C-terminal domain-containing protein	-
IF719_RS08010 (1613429)	1613429..1613746	-	318	WP_001103933.1	DUF1474 family protein	Toxin
IF719_RS08015 (1613749)	1613749..1613958	-	210	WP_001058488.1	hypothetical protein	Antitoxin
IF719_RS08020 (1613951)	1613951..1614097	-	147	WP_000780975.1	hypothetical protein	-
IF719_RS08025 (1614144)	1614144..1614404	-	261	WP_001147662.1	hypothetical protein	-
IF719_RS08030 (1614406)	1614406..1614603	-	198	WP_000466891.1	helix-turn-helix transcriptional regulator	-
IF719_RS08035 (1614747)	1614747..1615370	+	624	WP_000431539.1	helix-turn-helix transcriptional regulator	-
IF719_RS08040 (1615466)	1615466..1616602	+	1137	WP_000592973.1	site-specific integrase	-
IF719_RS08045 (1616696)	1616696..1618237	-	1542	WP_000424963.1	glutamine-hydrolyzing GMP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sell / sec	1595861..1621034	25173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12511.13 Da
• Isoelectric Point: 4.6364

>T175158 WP_001103933.1 NZ_CP062416:c1613746-1613429 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKEKINDVAIKHGWFVEDKFVKNELETKQEHINFSASYLEHRIQNEHTVELLQVYLKEFGELIQKF
HEIEKASSENFGEESDDAKKLKITE

Nucleotide

• Length: 318 bp

>T175158 NZ_CP081785:23070-23381 [Escherichia coli]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8287.31 Da
• Isoelectric Point: 4.2820

>AT175158 WP_001058488.1 NZ_CP062416:c1613958-1613749 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSIMNNFESYEHTEYVDGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT175158 NZ_CP081785:23378-23797 [Escherichia coli]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTTCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: K7ZRX2

Antitoxin

• AlphaFold DB: A0A2X2M557