Detailed information of TA system TA174451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1229682..1230211
• Replicon: chromosome
• Accession: NZ_CP062344
• Organism: Staphylococcus aureus strain NAS_AN_265

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: IF740_RS06225
• Protein ID: WP_001103939.1
• Coordinates: 1229682..1229999 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: IF740_RS06230
• Protein ID: WP_001058494.1
• Coordinates: 1230002..1230211 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF740_RS14090 (1225306)	1225306..1225440	-	135	WP_256094151.1	hypothetical protein	-
IF740_RS06200 (1225688)	1225688..1226329	-	642	WP_001019808.1	hypothetical protein	-
IF740_RS06205 (1226326)	1226326..1226610	-	285	WP_000998185.1	hypothetical protein	-
IF740_RS06210 (1226612)	1226612..1226974	-	363	WP_001039170.1	hypothetical protein	-
IF740_RS06215 (1227275)	1227275..1228732	-	1458	WP_000390453.1	virulence-associated E family protein	-
IF740_RS06220 (1228749)	1228749..1229618	-	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
IF740_RS06225 (1229682)	1229682..1229999	-	318	WP_001103939.1	DUF1474 family protein	Toxin
IF740_RS06230 (1230002)	1230002..1230211	-	210	WP_001058494.1	hypothetical protein	Antitoxin
IF740_RS06235 (1230204)	1230204..1230350	-	147	WP_000784885.1	hypothetical protein	-
IF740_RS06240 (1230362)	1230362..1230580	-	219	WP_000163544.1	helix-turn-helix transcriptional regulator	-
IF740_RS06245 (1230616)	1230616..1230819	-	204	WP_001045296.1	transcriptional regulator	-
IF740_RS06250 (1230955)	1230955..1231758	+	804	WP_000358990.1	helix-turn-helix transcriptional regulator	-
IF740_RS06255 (1231748)	1231748..1232854	+	1107	WP_000149511.1	tyrosine-type recombinase/integrase	-
IF740_RS06265 (1233417)	1233417..1233881	-	465	WP_001085186.1	SsrA-binding protein SmpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sell / sec	1213745..1236275	22530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T174451 WP_001103939.1 NZ_CP062344:c1229999-1229682 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T174451 NZ_CP081009:3175285-3175581 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT174451 WP_001058494.1 NZ_CP062344:c1230211-1230002 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT174451 NZ_CP081009:3175583-3175978 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59