Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2071796..2072627 Replicon chromosome
Accession NZ_CP062247
Organism Escherichia coli strain AML002_ev03

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag IHN48_RS10245 Protein ID WP_000854814.1
Coordinates 2072253..2072627 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag IHN48_RS10240 Protein ID WP_001285584.1
Coordinates 2071796..2072164 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN48_RS10225 2069463..2070995 + 1533 WP_001350525.1 hypothetical protein -
IHN48_RS10230 2070992..2071438 + 447 WP_000187523.1 RadC family protein -
IHN48_RS10235 2071501..2071722 + 222 WP_000692323.1 DUF987 domain-containing protein -
IHN48_RS10240 2071796..2072164 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
IHN48_RS10245 2072253..2072627 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
IHN48_RS10250 2072624..2072818 + 195 WP_000988600.1 hypothetical protein -
IHN48_RS10255 2072831..2072944 + 114 WP_001161660.1 DUF957 domain-containing protein -
IHN48_RS10260 2073233..2073361 - 129 Protein_2004 transposase domain-containing protein -
IHN48_RS10265 2073433..2073615 + 183 WP_001016348.1 hypothetical protein -
IHN48_RS10270 2073716..2074045 - 330 WP_000450409.1 DUF496 family protein -
IHN48_RS10275 2074217..2075275 - 1059 WP_001200891.1 FUSC family protein -
IHN48_RS10280 2075473..2075946 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
IHN48_RS10285 2076065..2077231 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T173999 WP_000854814.1 NZ_CP062247:2072253-2072627 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T173999 NZ_CP080470:c1091810-1091592 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT173999 WP_001285584.1 NZ_CP062247:2071796-2072164 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT173999 NZ_CP080470:c1092212-1091838 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References