Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3227086..3227813 Replicon chromosome
Accession NZ_CP062245
Organism Escherichia coli strain AML003_ev01

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IHN50_RS15650 Protein ID WP_000550189.1
Coordinates 3227499..3227813 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IHN50_RS15645 Protein ID WP_000560266.1
Coordinates 3227086..3227502 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN50_RS15635 3222246..3224597 + 2352 WP_000695487.1 alpha-glucosidase -
IHN50_RS15640 3225023..3227041 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IHN50_RS15645 3227086..3227502 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IHN50_RS15650 3227499..3227813 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IHN50_RS15655 3228097..3229233 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IHN50_RS15660 3229318..3229821 + 504 WP_001333820.1 M48 family metallopeptidase -
IHN50_RS15665 3229898..3230590 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
IHN50_RS15670 3230669..3231655 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T173920 WP_000550189.1 NZ_CP062245:c3227813-3227499 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T173920 NZ_CP080400:425779-426354 [Dickeya fangzhongdai]
ATGACCCATACACCTTATCCTGTCGTGCTAGATGCGTGCGTAATTTATCCATCTCTCCTGCGGGATTTATTAATCCACTT
AGGACTAACAGGGCTGTATCAGCCTAAGTGGACGGCCATCATCGAGGATGAATGGCAACGCAATCTACTGCTGAATAGAC
CTGATTTAACCCCGGAGCAAATCTCGCGGACACGCGAGCTGATGAATCAGGCTATTCCTGATGCGATGATTACTGGGTTT
GAAGCGCTCATTCCAGGCATCACGCTCCCTGATCCTGATGATCAGCATGTGGTTGCAGCCGCCATTCGCAGTAACGCTGA
AATCATCGTTACGTTTAACCTGAAAGATTTTCCCCATCCCGAGATCGATAACTTTGGCATTGAAGCGCTACACCCGGATG
ATTTCATCACAGACCTGCTCGATTTGAATCAGGCGCTTGTGTTATCCGCAGTAAGCAGGCAGCGTCACAGCATGAGAAAA
CCGCCTAAATCGGTTGATGAGTATCTTGATGCGTTACTCCGGCAGAGCTTACCGCAAACAGTAAAAGAATTGAGTAAATT
CCACGCCCTGATTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT173920 WP_000560266.1 NZ_CP062245:c3227502-3227086 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT173920 NZ_CP080400:425318-425779 [Dickeya fangzhongdai]
ATGACAAACTCAATTCTTGATAGCCTGAGCCTTCCGGCACCAGGGGAGATTGAGGCGGCTGTTCGTGGACAAAGGGAGCT
TGCTGCCTATCTCTCCACAAAGATGGAAACACAAAAAATCTCGATTCAGGACGCAGATAACAACACTCGTCAGGTCGAAT
TACCGACGTCGGCACTGACATTATTGATGTCGATTTTAGGCGAGCTGGCTGTGGGTAACGCGGTTCAGGTTGTTCCTGTT
CATGCTGAATTAACGACACAGGAGGCAGCCAACATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTCTGGAGGATGG
GAAACTACCTTTTCATAAAACTGGTCGCCACCGCCGTGTGCTTTTCGCTGACCTGATGGACTATAAACACCAGAGGGATA
ATGAAAGCAGTAGAGCAATGCAGGCGTTGGCAGATCATAGCCAAGAGCTTGGATTGTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References