Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 13947..14472 Replicon plasmid pB
Accession NZ_CP062140
Organism Klebsiella quasipneumoniae strain 203 19001

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag IG177_RS26060 Protein ID WP_001568026.1
Coordinates 13947..14252 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag IG177_RS26065 Protein ID WP_001568025.1
Coordinates 14254..14472 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IG177_RS26040 10417..11685 + 1269 WP_019705993.1 ISL3 family transposase -
IG177_RS26045 12020..13036 - 1017 WP_020315256.1 hypothetical protein -
IG177_RS26050 13033..13356 - 324 WP_022644730.1 hypothetical protein -
IG177_RS26055 13383..13778 - 396 WP_072158608.1 hypothetical protein -
IG177_RS26060 13947..14252 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
IG177_RS26065 14254..14472 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IG177_RS26070 14642..14899 - 258 Protein_13 hypothetical protein -
IG177_RS26075 15059..15679 + 621 WP_042015168.1 hypothetical protein -
IG177_RS26080 15943..17316 + 1374 WP_042015169.1 hypothetical protein -
IG177_RS26085 17434..18357 - 924 WP_192242977.1 J domain-containing protein -
IG177_RS26090 18439..18693 - 255 WP_042015188.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 10417..11685 1268
- inside Non-Mobilizable plasmid - - 1..87654 87654


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T173407 WP_001568026.1 NZ_CP062140:c14252-13947 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T173407 NZ_CP080163:3460396-3460503 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT173407 WP_001568025.1 NZ_CP062140:c14472-14254 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT173407 NZ_CP080163:c3460346-3460283 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References