Detailed information of TA system TA173352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4839295..4840976
• Replicon: chromosome
• Accession: NZ_CP061975
• Organism: Klebsiella pneumoniae strain IR5086

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A6TH06
• Locus tag: H4I67_RS23565
• Protein ID: WP_015959269.1
• Coordinates: 4839295..4840170 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: H4I67_RS23570
• Protein ID: WP_004177809.1
• Coordinates: 4840218..4840976 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4I67_RS23545	4834601..4836121	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
H4I67_RS23550	4836118..4837116	+	999	WP_002885172.1	ribose ABC transporter permease	-
H4I67_RS23555	4837147..4838088	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
H4I67_RS23560	4838163..4839170	+	1008	WP_015959270.1	carbohydrate kinase family protein	-
H4I67_RS23565	4839295..4840170	-	876	WP_015959269.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H4I67_RS23570	4840218..4840976	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
H4I67_RS23575	4841062..4841790	-	729	WP_032413197.1	response regulator transcription factor	-
H4I67_RS23580	4842063..4844210	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
H4I67_RS23585	4844207..4844350	-	144	WP_162897205.1	hypothetical protein	-
H4I67_RS23590	4844788..4845474	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32990.17 Da
• Isoelectric Point: 5.4637

>T173352 WP_015959269.1 NZ_CP061975:c4840170-4839295 [Klebsiella pneumoniae]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQAGMSAVLQANRSDE
MRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKSCITQQDVDFLLSFK
RGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T173352 NZ_CP080146:4203889-4203996 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT173352 WP_004177809.1 NZ_CP061975:c4840976-4840218 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT173352 NZ_CP080146:c4203841-4203775 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A6TH06

Antitoxin

• AlphaFold DB: A6TH05