173224

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 159171..159693 Replicon plasmid unnamed1
Accession NZ_CP061949
Organism Klebsiella pneumoniae strain IR5065

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag H4I82_RS27325 Protein ID WP_004181778.1
Coordinates 159409..159693 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A5Q9LM53
Locus tag H4I82_RS27320 Protein ID WP_025368601.1
Coordinates 159171..159419 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I82_RS27300 154380..155201 - 822 WP_004026535.1 hypothetical protein -
H4I82_RS27305 155263..155616 - 354 WP_004181774.1 hypothetical protein -
H4I82_RS27310 155761..156747 - 987 WP_025368599.1 hypothetical protein -
H4I82_RS27315 157081..158880 - 1800 WP_025368600.1 ATP-dependent helicase -
H4I82_RS27320 159171..159419 + 249 WP_025368601.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H4I82_RS27325 159409..159693 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H4I82_RS27330 159923..161149 + 1227 WP_025368602.1 transposase -
H4I82_RS27335 161435..161860 - 426 WP_040120489.1 IS200/IS605 family transposase -
H4I82_RS27340 161897..163123 + 1227 WP_025368604.1 transposase -
H4I82_RS27345 163189..164315 - 1127 Protein_170 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia iutA / iutA / iucD / iucC / iucC / iucB / iucA / iucA / rmpA / rmpA2 / iutA 1..295331 295331


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T173224 WP_004181778.1 NZ_CP061949:159409-159693 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T173224 NZ_CP080120:2400277-2400379 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8906.12 Da        Isoelectric Point: 3.8206

>AT173224 WP_025368601.1 NZ_CP061949:159171-159419 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT173224 NZ_CP080120:c2400417-2400149 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LM53

References