Detailed information of TA system TA173190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 158175..158832
• Replicon: plasmid pBD-50-Km_VIM-1
• Accession: NZ_CP061931
• Organism: Klebsiella michiganensis strain BD-50-Km

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: IE970_RS31065
• Protein ID: WP_000270043.1
• Coordinates: 158175..158525 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IE970_RS31070
• Protein ID: WP_000124640.1
• Coordinates: 158530..158832 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE970_RS31030	154649..155146	-	498	WP_000062185.1	hypothetical protein	-
IE970_RS31035	155149..155637	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
IE970_RS31040	155734..156069	+	336	WP_000683477.1	hypothetical protein	-
IE970_RS31045	156084..156554	-	471	WP_001281821.1	hypothetical protein	-
IE970_RS31050	156547..156918	-	372	WP_000516918.1	hypothetical protein	-
IE970_RS31055	156929..157123	-	195	WP_000343597.1	hypothetical protein	-
IE970_RS31060	157464..158012	-	549	WP_001061573.1	hypothetical protein	-
IE970_RS31065	158175..158525	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IE970_RS31070	158530..158832	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
IE970_RS31075	158859..159152	-	294	WP_001239998.1	hypothetical protein	-
IE970_RS31080	159241..159513	-	273	WP_001043046.1	HU family DNA-binding protein	-
IE970_RS31085	159571..160098	-	528	WP_001210659.1	thermonuclease family protein	-
IE970_RS31090	160101..160406	-	306	WP_171820680.1	hypothetical protein	-
IE970_RS31095	160318..161175	-	858	WP_001167036.1	hypothetical protein	-
IE970_RS31100	161162..161392	-	231	WP_000972665.1	hypothetical protein	-
IE970_RS31105	161392..161910	-	519	WP_000210757.1	nitrite reductase	-
IE970_RS31110	161907..162353	-	447	WP_000919343.1	hypothetical protein	-
IE970_RS31115	162353..162712	-	360	WP_000422769.1	hypothetical protein	-
IE970_RS31120	162770..163198	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qnrA1 / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / ant(2'')-Ia / blaSHV-2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / mph(A) / aac(6')-IIc / blaVIM-1	-	1..196307	196307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T173190 WP_000270043.1 NZ_CP061931:158175-158525 [Klebsiella michiganensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T173190 NZ_CP080119:465289-466182 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCGATGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTGATCTTTTAAGATTATTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGTAGTACCGCAT
TATCAAAAGCAGGTATCTCGCTAAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTATTCATAATTCTGACATTTT
CAGCTTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCACAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACTCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCCCTTGTAACGGAGTTAACGGGTAAAGGTACACCTATAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTTC
TGGTACGTCACATTTATGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATTATCCTGTACACAAATTGCGCTTTCAACGATTTGTTAACCCGATGGTATTTGGTAATA
CACAGATTAACTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT173190 WP_000124640.1 NZ_CP061931:158530-158832 [Klebsiella michiganensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT173190 NZ_CP080119:464901-465296 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAACGTTATGTGTTCACCCGGGATGATTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGTAACATCATCACTGGGAAAATCATGCCCGCAGCGCCTGGGGGCTCTGCTGCAGTAATTACAGAG
GCCCTGGAGCGCCTGAATATCAGATACCGTCTGGCAGGAGCAACGGAATGCTACAATAAAGGTCAGTCCACACAAATTCC
TGTATCTCTGGGGATAAAAACACCTTCTCGATTTAAAAGAGTGTTAATAGTAGGGAAAAGTAAAATCAATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9