Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3684302..3684920 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ID627_RS18165 Protein ID WP_001291435.1
Coordinates 3684702..3684920 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ID627_RS18160 Protein ID WP_000344800.1
Coordinates 3684302..3684676 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS18150 3679391..3680584 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ID627_RS18155 3680607..3683756 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ID627_RS18160 3684302..3684676 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ID627_RS18165 3684702..3684920 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ID627_RS18170 3685092..3685643 + 552 WP_000102564.1 maltose O-acetyltransferase -
ID627_RS18175 3685759..3686229 + 471 WP_000136192.1 YlaC family protein -
ID627_RS18180 3686393..3687943 + 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ID627_RS18185 3687985..3688338 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ID627_RS18190 3688743..3689195 - 453 WP_000566735.1 hypothetical protein -
ID627_RS18195 3689396..3689623 - 228 WP_000513895.1 DUF2188 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172969 WP_001291435.1 NZ_CP061764:3684702-3684920 [Escherichia coli O19:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T172969 NZ_CP080066:2344714-2346036 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT172969 WP_000344800.1 NZ_CP061764:3684302-3684676 [Escherichia coli O19:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT172969 NZ_CP080066:2344448-2344714 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References