Detailed information of TA system TA172934

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1179135..1179922
• Replicon: chromosome
• Accession: NZ_CP061758
• Organism: Escherichia coli O68:H12 strain 97.3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: IEC98_RS05615
• Protein ID: WP_032669201.1
• Coordinates: 1179135..1179512 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A6L5EE19
• Locus tag: IEC98_RS05620
• Protein ID: WP_023567998.1
• Coordinates: 1179563..1179922 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEC98_RS05585	1174512..1176764	-	2253	Protein_1099	hypothetical protein	-
IEC98_RS05600	1177502..1178350	-	849	WP_032669204.1	DUF4942 domain-containing protein	-
IEC98_RS05605	1178414..1178617	-	204	WP_032669203.1	DUF957 domain-containing protein	-
IEC98_RS05610	1178647..1179138	-	492	WP_032672023.1	hypothetical protein	-
IEC98_RS05615	1179135..1179512	-	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
IEC98_RS05620	1179563..1179922	-	360	WP_023567998.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IEC98_RS05625	1179946..1180167	-	222	WP_023567997.1	DUF987 domain-containing protein	-
IEC98_RS05630	1180188..1180667	-	480	WP_032669200.1	DNA repair protein RadC	-
IEC98_RS05635	1180679..1181122	-	444	WP_032669199.1	antirestriction protein	-
IEC98_RS05640	1181153..1181974	-	822	WP_032669198.1	DUF945 domain-containing protein	-
IEC98_RS05645	1182073..1182304	-	232	Protein_1109	DUF905 domain-containing protein	-
IEC98_RS05650	1182376..1182825	-	450	WP_032669197.1	hypothetical protein	-
IEC98_RS05655	1182822..1183277	-	456	WP_000754598.1	hypothetical protein	-
IEC98_RS05660	1183316..1183951	-	636	WP_020837117.1	hypothetical protein	-
IEC98_RS05665	1183948..1184661	-	714	WP_191115613.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T172934 WP_032669201.1 NZ_CP061758:c1179512-1179135 [Escherichia coli O68:H12]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T172934 NZ_CP080056:295786-296016 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13192.04 Da
• Isoelectric Point: 6.3122

>AT172934 WP_023567998.1 NZ_CP061758:c1179922-1179563 [Escherichia coli O68:H12]
MSNEIPTVNHDITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT172934 NZ_CP080056:296013-296372 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6

Antitoxin

• AlphaFold DB: A0A6L5EE19