Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 89729..90330 Replicon plasmid unnamed2
Accession NZ_CP061756
Organism Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)


Gene name doc Uniprot ID Q47172
Locus tag IEC99_RS25255 Protein ID WP_001694510.1
Coordinates 89729..90109 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IEC99_RS25260 Protein ID WP_001190712.1
Coordinates 90109..90330 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC99_RS25230 85170..86654 - 1485 WP_000124150.1 terminase -
IEC99_RS25235 86654..87847 - 1194 WP_032153803.1 hypothetical protein -
IEC99_RS25240 87933..88385 - 453 WP_060616011.1 hypothetical protein -
IEC99_RS25245 88474..89517 - 1044 WP_023356283.1 DUF968 domain-containing protein -
IEC99_RS25250 89545..89724 - 180 WP_163373566.1 PdcA protein -
IEC99_RS25255 89729..90109 - 381 WP_001694510.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IEC99_RS25260 90109..90330 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IEC99_RS25265 90513..92069 + 1557 WP_072643605.1 type I restriction-modification system subunit M -
IEC99_RS25270 92066..93289 + 1224 WP_191111788.1 restriction endonuclease subunit S -
IEC99_RS25275 93291..94490 + 1200 WP_163373564.1 anticodon nuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkF / mrkD / mrkC / mrkB / mrkA 1..115966 115966


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13616.35 Da        Isoelectric Point: 5.1514

>T172927 WP_001694510.1 NZ_CP061756:c90109-89729 [Escherichia coli O18ac:H14]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T172927 NZ_CP080029:3214943-3215194 [Streptomyces anulatus]
ATGCGGACCATGACCTACACCGAGTCCCGCGCCAAGTACGCCGAGACCCTGAGCGCGGTCGTGGACGACCGCGAGGAGGT
CGTGGTCACGCGTGCCGGGCATGAGCCCGTGGTGATAGTGGCGCTCGACGAGTACGAGTCCCTCAAGGAGACCGCGTACC
AGCTCCGGAGCCCGGAGAACGCCCGCCGTCTGCTGGCTTCGATCGATCGCCTGGAGAACGGCGGCGGCACGGTGCGGGAG
CTCGCGGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT172927 WP_001190712.1 NZ_CP061756:c90330-90109 [Escherichia coli O18ac:H14]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT172927 NZ_CP080029:3215191-3215451 [Streptomyces anulatus]
GTGAAGTTCGTGTGGGACCAGTCGTCCTGGGACGACTACGTCTGGTGGCGGAACCAGGATCGCAAGATCCTCAAGCGGAT
CAACACGCTGCTCCAGGACATCGCCCGGAACGGCAACGAGGGGATGGGCAAGCCGGAACCTCTGAAGCACGGCTTCCAGG
GGTACTGGTCGCGCCGCATCACCGACGAGCACCGGCTCATCTACAAGGTGGTCGACGACGAGGTCCGCGTCGCCGCCTGC
CGGTACCACTACGGCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A737M8C8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References