Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3836878..3837419 Replicon chromosome
Accession NZ_CP061754
Organism Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag IEC99_RS18740 Protein ID WP_000615983.1
Coordinates 3837141..3837419 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag IEC99_RS18735 Protein ID WP_000729703.1
Coordinates 3836878..3837138 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC99_RS18720 3832929..3835022 + 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -
IEC99_RS18725 3835246..3835743 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
IEC99_RS18730 3835919..3836668 - 750 WP_024192679.1 C40 family peptidase -
IEC99_RS18735 3836878..3837138 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
IEC99_RS18740 3837141..3837419 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
IEC99_RS18745 3837575..3838315 + 741 WP_001225679.1 murein L,D-transpeptidase -
IEC99_RS18750 3838286..3839053 - 768 WP_000333380.1 class II glutamine amidotransferase -
IEC99_RS18755 3839259..3839837 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T172925 WP_000615983.1 NZ_CP061754:3837141-3837419 [Escherichia coli O18ac:H14]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T172925 NZ_CP080013:c1856270-1855920 [Macrococcus caseolyticus]
ATGAAACGCGGAGATGTTTATTTAGCTGATCTGTCACCAGTGAAGGGGTCTGAACAAGGCGGTAAACGCCCCGTCGTTAT
TATTCAGAATAATATTGGGAATAAACATAGTCCTACAGTGATTGTAGCAGCAATTACTGCAAAGATTAATAAGGCGAAAA
TCCCCACCCATGTTGAAATTGGCAAGACAACGCATCGCTTAGAAAAGGATTCGGTAATACTGCTGGAACAAATTAGAACC
ATTGATAAGAATAGGTTGATTGATCATTTAACTGTGCTTGATAAGGCGAAGATGAAAGAAGTGGATACTGCACTTATAAT
TAGTTTGGGTTTATCAATCGATAGTGAATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT172925 WP_000729703.1 NZ_CP061754:3836878-3837138 [Escherichia coli O18ac:H14]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT172925 NZ_CP080013:c1856411-1856271 [Macrococcus caseolyticus]
ATGAGCCAGAAGCTAGAACAAATGCTGATAGAAGGTTATCAATCAATGGCAGAGATAAATTTATCGATAGCATCAGAACA
TTATGTATTAGAATGTGAAGTTACTGATAACTATCAGACATATACCTTTGATGGTGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References