Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1057470..1058053 Replicon chromosome
Accession NZ_CP061754
Organism Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IEC99_RS05105 Protein ID WP_000254738.1
Coordinates 1057718..1058053 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IEC99_RS05100 Protein ID WP_000581937.1
Coordinates 1057470..1057718 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC99_RS05090 1053809..1055110 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IEC99_RS05095 1055158..1057392 + 2235 WP_044864291.1 GTP diphosphokinase -
IEC99_RS05100 1057470..1057718 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IEC99_RS05105 1057718..1058053 + 336 WP_000254738.1 endoribonuclease MazF Toxin
IEC99_RS05110 1058124..1058915 + 792 Protein_1007 nucleoside triphosphate pyrophosphohydrolase -
IEC99_RS05115 1059143..1060780 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IEC99_RS05120 1060868..1062166 + 1299 WP_160183230.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T172909 WP_000254738.1 NZ_CP061754:1057718-1058053 [Escherichia coli O18ac:H14]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T172909 NZ_CP080007:c145048-144767 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT172909 WP_000581937.1 NZ_CP061754:1057470-1057718 [Escherichia coli O18ac:H14]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT172909 NZ_CP080007:c145272-145045 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGATGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References