Detailed information of TA system TA172907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 780394..781087
• Replicon: chromosome
• Accession: NZ_CP061754
• Organism: Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: IEC99_RS03795
• Protein ID: WP_000415584.1
• Coordinates: 780394..780690 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: IEC99_RS03800
• Protein ID: WP_000650107.1
• Coordinates: 780692..781087 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEC99_RS03765	775528..776720	+	1193	Protein_742	Hcp family type VI secretion system effector	-
IEC99_RS03770	776727..777059	+	333	WP_000914691.1	DUF2645 family protein	-
IEC99_RS03775	777105..778454	-	1350	WP_000673354.1	two-component system sensor histidine kinase QseC	-
IEC99_RS03780	778451..779110	-	660	WP_191028008.1	two-component system response regulator QseB	-
IEC99_RS03785	779262..779654	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
IEC99_RS03790	779707..780189	+	483	WP_000183494.1	transcriptional regulator	-
IEC99_RS03795	780394..780690	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
IEC99_RS03800	780692..781087	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
IEC99_RS03805	781220..782668	+	1449	WP_191028007.1	ABC transporter substrate-binding protein	-
IEC99_RS03810	782806..785064	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
IEC99_RS03815	785298..786035	+	738	WP_000965712.1	1-acylglycerol-3-phosphate O-acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T172907 WP_000415584.1 NZ_CP061754:780394-780690 [Escherichia coli O18ac:H14]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T172907 NZ_CP079993:4236879-4237343 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT172907 WP_000650107.1 NZ_CP061754:780692-781087 [Escherichia coli O18ac:H14]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT172907 NZ_CP079993:4236544-4236879 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65