Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4046044..4046831 Replicon chromosome
Accession NZ_CP061749
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2H9G3C1
Locus tag IED00_RS19635 Protein ID WP_001568519.1
Coordinates 4046454..4046831 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag IED00_RS19630 Protein ID WP_000066236.1
Coordinates 4046044..4046403 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS19590 4041740..4041886 + 147 WP_023563913.1 hypothetical protein -
IED00_RS19595 4041899..4042114 + 216 WP_041036768.1 hypothetical protein -
IED00_RS19600 4042228..4042638 + 411 WP_042851426.1 hypothetical protein -
IED00_RS19605 4042704..4043642 + 939 WP_077871856.1 hypothetical protein -
IED00_RS19610 4043731..4044549 + 819 WP_016236913.1 DUF945 domain-containing protein -
IED00_RS19615 4044817..4045287 + 471 WP_000131762.1 antirestriction protein -
IED00_RS19620 4045299..4045778 + 480 WP_000437750.1 DNA repair protein RadC -
IED00_RS19625 4045799..4046020 + 222 WP_000691981.1 DUF987 domain-containing protein -
IED00_RS19630 4046044..4046403 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IED00_RS19635 4046454..4046831 + 378 WP_001568519.1 TA system toxin CbtA family protein Toxin
IED00_RS19640 4046828..4047319 + 492 WP_000777682.1 hypothetical protein -
IED00_RS19645 4047351..4047554 + 204 WP_000413747.1 DUF957 domain-containing protein -
IED00_RS19650 4047635..4048480 + 846 WP_016236912.1 DUF4942 domain-containing protein -
IED00_RS19660 4048822..4049553 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
IED00_RS19665 4049618..4050085 + 468 WP_000917883.1 ribonuclease HI -
IED00_RS19670 4050082..4050804 - 723 WP_001295200.1 class I SAM-dependent methyltransferase -
IED00_RS19675 4050838..4051593 + 756 WP_001052721.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4028450..4051593 23143


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14211.00 Da        Isoelectric Point: 7.4209

>T172893 WP_001568519.1 NZ_CP061749:4046454-4046831 [Escherichia coli O15:H12]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKTTGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T172893 NZ_CP079993:c2149231-2149124 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT172893 WP_000066236.1 NZ_CP061749:4046044-4046403 [Escherichia coli O15:H12]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT172893 NZ_CP079993:2149278-2149344 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9G3C1

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References