Detailed information of TA system TA172875

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1950210..1951041
• Replicon: chromosome
• Accession: NZ_CP061749
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: IED00_RS09300
• Protein ID: WP_097719127.1
• Coordinates: 1950210..1950584 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0EA76
• Locus tag: IED00_RS09305
• Protein ID: WP_001285592.1
• Coordinates: 1950673..1951041 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS09260	1945606..1946772	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
IED00_RS09265	1946891..1947364	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
IED00_RS09270	1947562..1948620	+	1059	WP_001200891.1	FUSC family protein	-
IED00_RS09275	1948792..1949121	+	330	WP_000450409.1	DUF496 family protein	-
IED00_RS09280	1949222..1949404	-	183	WP_001016348.1	hypothetical protein	-
IED00_RS09285	1949476..1949604	+	129	Protein_1828	transposase domain-containing protein	-
IED00_RS09290	1949893..1950006	-	114	WP_001161660.1	DUF957 domain-containing protein	-
IED00_RS09295	1950019..1950213	-	195	WP_000988600.1	hypothetical protein	-
IED00_RS09300	1950210..1950584	-	375	WP_097719127.1	type IV toxin-antitoxin system toxin CbtA	Toxin
IED00_RS09305	1950673..1951041	-	369	WP_001285592.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
IED00_RS09310	1951115..1951336	-	222	WP_000692323.1	DUF987 domain-containing protein	-
IED00_RS09315	1951399..1951875	-	477	WP_001186726.1	RadC family protein	-
IED00_RS09320	1951891..1952370	-	480	WP_000860086.1	antirestriction protein	-
IED00_RS09325	1952452..1953270	-	819	WP_047090156.1	DUF945 domain-containing protein	-
IED00_RS09330	1953370..1953603	-	234	WP_001119729.1	DUF905 family protein	-
IED00_RS09335	1953682..1954137	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13945.03 Da
• Isoelectric Point: 7.7467

>T172875 WP_097719127.1 NZ_CP061749:c1950584-1950210 [Escherichia coli O15:H12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLCKYPEAK

Nucleotide

• Length: 375 bp

>T172875 NZ_CP079992:3594622-3595695 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13636.51 Da
• Isoelectric Point: 6.6249

>AT172875 WP_001285592.1 NZ_CP061749:c1951041-1950673 [Escherichia coli O15:H12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT172875 NZ_CP079992:3595688-3596059 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H0EA76