Detailed information of TA system TA172870

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 877514..878214
• Replicon: chromosome
• Accession: NZ_CP061749
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: IED00_RS04285
• Protein ID: WP_016240265.1
• Coordinates: 877514..877798 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A794RE17
• Locus tag: IED00_RS04290
• Protein ID: WP_016240264.1
• Coordinates: 877888..878214 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS04270	872643..873340	-	698	Protein_846	IS1 family transposase	-
IED00_RS04275	873396..874769	+	1374	Protein_847	lactate permease LctP family transporter	-
IED00_RS04280	875254..877551	+	2298	Protein_848	DUF4092 domain-containing protein	-
IED00_RS04285	877514..877798	-	285	WP_016240265.1	TA system toxin CbtA family protein	Toxin
IED00_RS04290	877888..878214	-	327	WP_016240264.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
IED00_RS04295	878233..878454	-	222	WP_016239820.1	DUF987 domain-containing protein	-
IED00_RS04300	878463..878939	-	477	WP_016243826.1	RadC family protein	-
IED00_RS04305	878955..879413	-	459	WP_000211838.1	antirestriction protein	-
IED00_RS04310	879511..879750	-	240	WP_000194654.1	DUF905 domain-containing protein	-
IED00_RS04315	879845..880549	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IED00_RS04320	880911..882593	+	1683	WP_061351512.1	MCP four helix bundle domain-containing protein	-
IED00_RS04325	882693..882944	+	252	WP_191111663.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	872643..873020	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-84)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10591.11 Da
• Isoelectric Point: 5.0063

>T172870 WP_016240265.1 NZ_CP061749:c877798-877514 [Escherichia coli O15:H12]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTLADRYLLPTC

Nucleotide

• Length: 285 bp

>T172870 NZ_CP079992:c2500261-2499974 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12332.00 Da
• Isoelectric Point: 7.0273

>AT172870 WP_016240264.1 NZ_CP061749:c878214-877888 [Escherichia coli O15:H12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRTSITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT172870 NZ_CP079992:c2500500-2500261 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A794RE17