Detailed information of TA system TA172868

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 767971..768650
• Replicon: chromosome
• Accession: NZ_CP061749
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: IED00_RS03745
• Protein ID: WP_113073117.1
• Coordinates: 768309..768650 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: IED00_RS03740
• Protein ID: WP_006812013.1
• Coordinates: 767971..768288 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS03695	763408..764229	+	822	WP_042111319.1	DUF945 domain-containing protein	-
IED00_RS03700	764446..765147	+	702	WP_001581462.1	WYL domain-containing protein	-
IED00_RS03705	765188..765424	+	237	WP_032181217.1	hypothetical protein	-
IED00_RS03710	765424..765867	+	444	WP_001547764.1	phage transcriptional regulator	-
IED00_RS03715	765890..766357	+	468	WP_001547765.1	hypothetical protein	-
IED00_RS03720	766434..766673	+	240	WP_000194654.1	DUF905 domain-containing protein	-
IED00_RS03725	766771..767229	+	459	WP_000211838.1	antirestriction protein	-
IED00_RS03730	767245..767721	+	477	WP_000811693.1	RadC family protein	-
IED00_RS03735	767730..767951	+	222	WP_000691995.1	DUF987 domain-containing protein	-
IED00_RS03740	767971..768288	+	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IED00_RS03745	768309..768650	+	342	WP_113073117.1	type IV toxin-antitoxin system toxin YkfI	Toxin
IED00_RS03755	769012..769518	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
IED00_RS03760	769597..771438	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
IED00_RS03765	771633..773378	-	1746	WP_000918827.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsM / kpsT / kpsS / kpsC / kpsD / kpsE / kpsF	730587..768650	38063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12866.91 Da
• Isoelectric Point: 9.9671

>T172868 WP_113073117.1 NZ_CP061749:768309-768650 [Escherichia coli O15:H12]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T172868 NZ_CP079992:2363914-2364090 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT172868 WP_006812013.1 NZ_CP061749:767971-768288 [Escherichia coli O15:H12]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT172868 NZ_CP079992:2364136-2364552 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: R8WMM2