Detailed information of TA system TA172845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4948256..4948844
• Replicon: chromosome
• Accession: NZ_CP061723
• Organism: Pseudomonas putida strain ZXPA-20

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: R9VGK1
• Locus tag: ID616_RS22490
• Protein ID: WP_016488542.1
• Coordinates: 4948256..4948558 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ID616_RS22495
• Protein ID: WP_191086862.1
• Coordinates: 4948551..4948844 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID616_RS22470	4944435..4945328	-	894	WP_016488538.1	LysR family transcriptional regulator	-
ID616_RS22475	4945435..4946604	+	1170	WP_096426616.1	MFS transporter	-
ID616_RS22480	4946652..4947578	-	927	WP_016488540.1	DMT family transporter	-
ID616_RS22485	4947685..4948080	-	396	WP_016498447.1	hypothetical protein	-
ID616_RS22490	4948256..4948558	+	303	WP_016488542.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ID616_RS22495	4948551..4948844	+	294	WP_191086862.1	putative addiction module antidote protein	Antitoxin
ID616_RS22500	4948934..4949206	+	273	WP_060519159.1	hypothetical protein	-
ID616_RS22505	4949328..4950689	-	1362	WP_191086863.1	DEAD/DEAH box helicase	-
ID616_RS22510	4950793..4952094	+	1302	WP_145913837.1	mechanosensitive ion channel family protein	-
ID616_RS22515	4952203..4953138	-	936	WP_023047769.1	AEC family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4940502..4959511	19009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11379.12 Da
• Isoelectric Point: 10.8638

>T172845 WP_016488542.1 NZ_CP061723:4948256-4948558 [Pseudomonas putida]
MKKIESSSFRHWVTGLRDLSARARIISRINRLMEGLPGDVSPVGHGVSELKIHYGPGYRVYFHQTGSTFVILLCGGDKSS
QKRDIKVAHQILRSWRMQND

Nucleotide

• Length: 303 bp

>T172845 NZ_CP079991:c2777834-2777731 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10432.86 Da
• Isoelectric Point: 5.0495

>AT172845 WP_191086862.1 NZ_CP061723:4948551-4948844 [Pseudomonas putida]
MTETFSEFDPAAYLTTPEAIAQFMTDALETGDASYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSDHGNPTLKSFLT
VMKALGIDMTARPHLAG

Nucleotide

• Length: 294 bp

>AT172845 NZ_CP079991:2777693-2777964 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: R9VGK1