Detailed information of TA system TA172843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3990095..3990978
• Replicon: chromosome
• Accession: NZ_CP061723
• Organism: Pseudomonas putida strain ZXPA-20

Toxin (Protein)

• Gene name: -
• Uniprot ID: R9VG37
• Locus tag: ID616_RS18215
• Protein ID: WP_016487683.1
• Coordinates: 3990095..3990532 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R9VFG2
• Locus tag: ID616_RS18220
• Protein ID: WP_016487684.1
• Coordinates: 3990529..3990978 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID616_RS18190	3985590..3985973	-	384	WP_191087892.1	hypothetical protein	-
ID616_RS18195	3985975..3986355	-	381	WP_191086726.1	hypothetical protein	-
ID616_RS18200	3986444..3987673	-	1230	WP_016487680.1	acyl-CoA dehydrogenase	-
ID616_RS18205	3987813..3988739	+	927	WP_060517277.1	LysR family transcriptional regulator	-
ID616_RS18210	3988933..3990087	+	1155	WP_016487682.1	aminotransferase class V-fold PLP-dependent enzyme	-
ID616_RS18215	3990095..3990532	-	438	WP_016487683.1	RES family NAD+ phosphorylase	Toxin
ID616_RS18220	3990529..3990978	-	450	WP_016487684.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
ID616_RS18225	3991084..3991860	-	777	WP_095115508.1	SDR family oxidoreductase	-
ID616_RS18230	3992004..3992924	-	921	WP_191086727.1	LysR family transcriptional regulator	-
ID616_RS18235	3993217..3993939	-	723	WP_191086728.1	hypothetical protein	-
ID616_RS18240	3994019..3994318	-	300	WP_016487688.1	DUF3077 domain-containing protein	-
ID616_RS18245	3994518..3995141	+	624	WP_016487689.1	glutathione S-transferase	-
ID616_RS18250	3995248..3995385	+	138	WP_157754405.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16005.35 Da
• Isoelectric Point: 6.2187

>T172843 WP_016487683.1 NZ_CP061723:c3990532-3990095 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPSGAMLEVLVHLEIDPEDFPTTMRLIRIELPDTVSQAQLPALQ
PGWSAQPELTRALGNRFLDDCSALLLPVPSAIMPSTINYLFNPRHAQAQRAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T172843 NZ_CP079991:c2446803-2445481 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17096.64 Da
• Isoelectric Point: 6.1089

>AT172843 WP_016487684.1 NZ_CP061723:c3990978-3990529 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLEIPELASDFEIHTRITHGFAATWLVKLTERGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEADKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT172843 NZ_CP079991:c2447069-2446803 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: R9VG37

Antitoxin

• AlphaFold DB: R9VFG2