Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5803..6328 Replicon plasmid pCRE-085-1
Accession NZ_CP061400
Organism Klebsiella pneumoniae strain NU-CRE085

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag HCV82_RS26930 Protein ID WP_001568026.1
Coordinates 5803..6108 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HCV82_RS26935 Protein ID WP_001568025.1
Coordinates 6110..6328 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV82_RS26900 1519..2145 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HCV82_RS26905 2265..2444 + 180 WP_006788217.1 hypothetical protein -
HCV82_RS26910 2884..3678 - 795 WP_004197635.1 site-specific integrase -
HCV82_RS26915 3876..4892 - 1017 WP_020315256.1 hypothetical protein -
HCV82_RS26920 4889..5212 - 324 WP_022644730.1 hypothetical protein -
HCV82_RS26925 5239..5634 - 396 WP_017899885.1 hypothetical protein -
HCV82_RS26930 5803..6108 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
HCV82_RS26935 6110..6328 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HCV82_RS26940 6881..7570 + 690 WP_004193995.1 hypothetical protein -
HCV82_RS26945 7602..8291 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
HCV82_RS26950 8728..8997 + 270 WP_020804424.1 hypothetical protein -
HCV82_RS26955 8985..9560 + 576 WP_020317495.1 hypothetical protein -
HCV82_RS26960 9591..10085 + 495 WP_020802777.1 hypothetical protein -
HCV82_RS26965 10129..10497 + 369 WP_004197807.1 hypothetical protein -
HCV82_RS26970 10531..10734 + 204 WP_004197808.1 hemolysin expression modulator Hha -
HCV82_RS26975 10748..10951 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..128320 128320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T172627 WP_001568026.1 NZ_CP061400:c6108-5803 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T172627 NZ_CP079827:c3947745-3947308 [Pseudomonas sp. HD6515]
GTGATTTTGTGGCGAATCAGCGCCTATACAGACTTGAGCGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGGCGGCCGGTGGTGTATGCCGCCACCAGCCCTCCCGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCGACCACCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCACAGTTGCCCGCTTTGCAG
CCCGGCTGGTCTGCTCACCCTGAGTTGACCCGTACGCTGGGCAATCGCTTTCTGGATGACGGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACCCCGGACAGCCGCCTGTTCTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT172627 WP_001568025.1 NZ_CP061400:c6328-6110 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT172627 NZ_CP079827:c3948191-3947742 [Pseudomonas sp. HD6515]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCTTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGTGACTTCGAGATTCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACCGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATCCCGCTGCGCACCCTCAAATCCCGTATCGAGCGCGACCAACCGCTGACC
GTGGATGAGAGCGATCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCTGTGTTCGGTGAAGCGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCCAAGGAGCGTTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References