Detailed information of TA system TA172419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2234245..2235833
• Replicon: chromosome
• Accession: NZ_CP061339
• Organism: Escherichia coli strain M00057

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: IC755_RS10685
• Protein ID: WP_001125415.1
• Coordinates: 2234245..2235567 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IC755_RS10690
• Protein ID: WP_001296726.1
• Coordinates: 2235567..2235833 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC755_RS10645	2229279..2229851	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
IC755_RS10650	2230002..2230193	-	192	WP_001469139.1	amidohydrolase family protein	-
IC755_RS10655	2230144..2230563	-	420	WP_001322416.1	amidohydrolase family protein	-
IC755_RS10660	2230523..2230678	-	156	WP_001322415.1	hypothetical protein	-
IC755_RS10665	2230781..2231728	-	948	WP_000854796.1	aldo/keto reductase	-
IC755_RS10670	2231802..2232491	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
IC755_RS10675	2232500..2232808	-	309	WP_001221046.1	hypothetical protein	-
IC755_RS10680	2232948..2233847	+	900	WP_001057776.1	LysR family transcriptional regulator	-
IC755_RS10685	2234245..2235567	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IC755_RS10690	2235567..2235833	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IC755_RS10695	2236044..2237444	-	1401	WP_001376867.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T172419 WP_001125415.1 NZ_CP061339:c2235567-2234245 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T172419 NZ_CP079752:c4830987-4830700 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT172419 WP_001296726.1 NZ_CP061339:c2235833-2235567 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT172419 NZ_CP079752:c4830713-4830408 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAATGCGATTTTAAGAGAAGCGATGATTCGCGAGCAGAATAAGAAATCGTAG

Structures

Toxin

• AlphaFold DB: U9YVT8