Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3077825..3078479 Replicon chromosome
Accession NZ_CP061206
Organism Escherichia coli strain DA61218

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ICI53_RS14510 Protein ID WP_000244781.1
Coordinates 3078072..3078479 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ICI53_RS14505 Protein ID WP_000354046.1
Coordinates 3077825..3078091 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI53_RS14485 3073913..3075346 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
ICI53_RS14490 3075391..3075702 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
ICI53_RS14495 3075866..3076525 + 660 WP_000250274.1 hemolysin III family protein -
ICI53_RS14500 3076602..3077582 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
ICI53_RS14505 3077825..3078091 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ICI53_RS14510 3078072..3078479 + 408 WP_000244781.1 protein YgfX Toxin
ICI53_RS14515 3078519..3079040 - 522 WP_001055874.1 flavodoxin FldB -
ICI53_RS14520 3079152..3080048 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ICI53_RS14525 3080073..3080783 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ICI53_RS14530 3080789..3082522 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T172242 WP_000244781.1 NZ_CP061206:3078072-3078479 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172242 NZ_CP079673:2774118-2774651 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172242 WP_000354046.1 NZ_CP061206:3077825-3078091 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172242 NZ_CP079673:2773841-2774107 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References