Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1579056..1579888 Replicon chromosome
Accession NZ_CP061206
Organism Escherichia coli strain DA61218

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2R9V8K2
Locus tag ICI53_RS07385 Protein ID WP_001468432.1
Coordinates 1579514..1579888 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID Q1R2L5
Locus tag ICI53_RS07380 Protein ID WP_001285575.1
Coordinates 1579056..1579424 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICI53_RS07345 1574949..1575629 + 681 WP_001282919.1 WYL domain-containing protein -
ICI53_RS07350 1575777..1576454 + 678 WP_001097301.1 hypothetical protein -
ICI53_RS07355 1576460..1576693 + 234 WP_001278283.1 DUF905 family protein -
ICI53_RS07360 1576783..1577601 + 819 WP_001175160.1 DUF945 domain-containing protein -
ICI53_RS07365 1577692..1578177 + 486 WP_000213697.1 antirestriction protein -
ICI53_RS07370 1578192..1578668 + 477 WP_001298074.1 RadC family protein -
ICI53_RS07375 1578755..1578976 + 222 WP_000692350.1 DUF987 domain-containing protein -
ICI53_RS07380 1579056..1579424 + 369 WP_001285575.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ICI53_RS07385 1579514..1579888 + 375 WP_001468432.1 TA system toxin CbtA family protein Toxin
ICI53_RS07390 1579885..1580373 + 489 WP_000777545.1 hypothetical protein -
ICI53_RS07395 1580385..1580582 + 198 WP_000839281.1 DUF957 domain-containing protein -
ICI53_RS07400 1580679..1580966 + 288 Protein_1444 DUF4942 domain-containing protein -
ICI53_RS07405 1581038..1582042 + 1005 WP_001620137.1 IS110-like element ISSfl8 family transposase -
ICI53_RS07410 1582324..1582605 + 282 Protein_1446 DUF4942 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1560629..1592705 32076
- flank IS/Tn - - 1581038..1582042 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14102.04 Da        Isoelectric Point: 7.3526

>T172236 WP_001468432.1 NZ_CP061206:1579514-1579888 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T172236 NZ_CP079673:c99103-99001 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13709.52 Da        Isoelectric Point: 6.6258

>AT172236 WP_001285575.1 NZ_CP061206:1579056-1579424 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT172236 NZ_CP079673:98994-99139 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2R9V8K2

Antitoxin

Source ID Structure
AlphaFold DB A0A454ABF5

References