Detailed information of TA system TA172235

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1305796..1306622
• Replicon: chromosome
• Accession: NZ_CP061206
• Organism: Escherichia coli strain DA61218

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: ICI53_RS06150
• Protein ID: WP_000854683.1
• Coordinates: 1305796..1306170 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ICI53_RS06155
• Protein ID: WP_024186744.1
• Coordinates: 1306260..1306622 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI53_RS06130	1303325..1304398	-	1074	WP_001327207.1	integrase arm-type DNA-binding domain-containing protein	-
ICI53_RS06135	1304862..1305014	-	153	WP_001555250.1	hypothetical protein	-
ICI53_RS06140	1305099..1305296	-	198	WP_000839235.1	DUF957 domain-containing protein	-
ICI53_RS06145	1305308..1305799	-	492	WP_000976831.1	hypothetical protein	-
ICI53_RS06150	1305796..1306170	-	375	WP_000854683.1	TA system toxin CbtA family protein	Toxin
ICI53_RS06155	1306260..1306622	-	363	WP_024186744.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ICI53_RS06160	1306701..1306922	-	222	WP_000692312.1	DUF987 domain-containing protein	-
ICI53_RS06165	1306991..1307467	-	477	WP_001186708.1	RadC family protein	-
ICI53_RS06170	1307483..1307968	-	486	WP_000206667.1	antirestriction protein	-
ICI53_RS06175	1308060..1308878	-	819	WP_001234592.1	DUF945 domain-containing protein	-
ICI53_RS06180	1309218..1310288	-	1071	WP_000102675.1	patatin-like phospholipase family protein	-
ICI53_RS06185	1310285..1311190	-	906	WP_000203561.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	1283733..1320230	36497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (16-115)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13993.03 Da
• Isoelectric Point: 8.2905

>T172235 WP_000854683.1 NZ_CP061206:c1306170-1305796 [Escherichia coli]
MKTLPDTHIREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTQFADERVIELHIEAGISLCDAVNFLVEKYALVRTGQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T172235 NZ_CP079670:58923-59405 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13354.17 Da
• Isoelectric Point: 6.6247

>AT172235 WP_024186744.1 NZ_CP061206:c1306622-1306260 [Escherichia coli]
VSDTLPGTTHPDNNRPWWGLPCTVMPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGEL
NPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 363 bp

>AT172235 NZ_CP079670:58669-58935 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA