Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3622700..3623318 Replicon chromosome
Accession NZ_CP060987
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9212_RS17610 Protein ID WP_001291435.1
Coordinates 3623100..3623318 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9212_RS17605 Protein ID WP_000344800.1
Coordinates 3622700..3623074 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9212_RS17595 (3617789) 3617789..3618982 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9212_RS17600 (3619005) 3619005..3622154 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9212_RS17605 (3622700) 3622700..3623074 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9212_RS17610 (3623100) 3623100..3623318 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9212_RS17615 (3623490) 3623490..3624041 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9212_RS17620 (3624157) 3624157..3624627 + 471 WP_000136192.1 YlaC family protein -
H9212_RS17625 (3624791) 3624791..3626341 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9212_RS17630 (3626383) 3626383..3626736 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9212_RS17640 (3627115) 3627115..3627426 + 312 WP_000409911.1 MGMT family protein -
H9212_RS17645 (3627457) 3627457..3628029 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171909 WP_001291435.1 NZ_CP060987:3623100-3623318 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171909 NZ_CP078509:c2282259-2281945 [Stutzerimonas stutzeri]
ATGATCTTTATCGAGACACCGATCTTCACCAAGCGTCTGCGGGATCTGCTTAGCGATGACAGCTACGCGGAGTTTCAGCG
ACAACTGGCTGACCGGCCGGACATGGGCGATGTGATTGAAGGTACTGGCGGCATTCGCAAGGTTCGCGTTGCGTCTAGCG
GTCACGGCAAGCGAGGCGGGTCCAGGGTCATCTACTACCACTTCACGGCGGCTTCGCAGATCGCATTGCTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGACGAGCGCAAGGTGCTCAAGCAAATCATCGAGCGGTGGAGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171909 WP_000344800.1 NZ_CP060987:3622700-3623074 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171909 NZ_CP078509:c2281939-2281649 [Stutzerimonas stutzeri]
ATGAGCAAATTCTTTGAAGACCTTCTGGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAACGTCAACCCTCGCG
GGAATTCGTCGTCGATTCGCTGCAGGTGAAAGAGATCCGCAAAGCAACTGGTCTGACCCAGGCCAAGTTCGCGGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGCCGACGCGAGCCGACCGGACCGGCAAAGGCGCTGCTGCGC
GCCATCCATAACGATCCGAAGCACGTCATCCAGGCGCTTTCTTCCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References