Detailed information of TA system TA171908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2591898..2592536
• Replicon: chromosome
• Accession: NZ_CP060987
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9212_RS12610
• Protein ID: WP_001447010.1
• Coordinates: 2592360..2592536 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9212_RS12605
• Protein ID: WP_001270286.1
• Coordinates: 2591898..2592314 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9212_RS12585 (2587050)	2587050..2587991	-	942	WP_001251304.1	ABC transporter permease	-
H9212_RS12590 (2587992)	2587992..2589005	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9212_RS12595 (2589023)	2589023..2590168	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9212_RS12600 (2590413)	2590413..2591819	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H9212_RS12605 (2591898)	2591898..2592314	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9212_RS12610 (2592360)	2592360..2592536	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9212_RS12615 (2592758)	2592758..2592988	+	231	WP_000494244.1	YncJ family protein	-
H9212_RS12620 (2593080)	2593080..2595041	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9212_RS12625 (2595114)	2595114..2595650	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9212_RS12630 (2595742)	2595742..2596917	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171908 WP_001447010.1 NZ_CP060987:c2592536-2592360 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171908 NZ_CP078508:c1341613-1341248 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATAGATACCAATGTACGACAATGGTTAGAAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAAT
AAAAACGGGGATTTTACTTAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATGATGTAT
TAAGCGTTTATCAAGCTAAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAA
ATGGATTTAAACGATGCTTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTT
CAACCGTTGCGATATACGTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171908 WP_001270286.1 NZ_CP060987:c2592314-2591898 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171908 NZ_CP078508:c1341963-1341676 [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8