Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3013048..3013589 Replicon chromosome
Accession NZ_CP060950
Organism Escherichia coli strain EC9

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag H9196_RS14440 Protein ID WP_000615976.1
Coordinates 3013048..3013326 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H9196_RS14445 Protein ID WP_000729704.1
Coordinates 3013329..3013589 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9196_RS14425 (3010630) 3010630..3011208 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
H9196_RS14430 (3011414) 3011414..3012181 + 768 WP_000333380.1 class II glutamine amidotransferase -
H9196_RS14435 (3012152) 3012152..3012892 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9196_RS14440 (3013048) 3013048..3013326 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9196_RS14445 (3013329) 3013329..3013589 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9196_RS14450 (3013775) 3013775..3014548 + 774 WP_000543899.1 C40 family peptidase -
H9196_RS14455 (3014605) 3014605..3014961 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H9196_RS14460 (3014954) 3014954..3015232 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H9196_RS14465 (3015337) 3015337..3017430 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
H9196_RS14470 (3017414) 3017414..3018553 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T171694 WP_000615976.1 NZ_CP060950:c3013326-3013048 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171694 NZ_CP078030:c3075-2779 [Acinetobacter variabilis]
ATGACCGAGATTAAACGTCTCCCTGAATTTGATGAATGGCTAGATAGCATTAAAGACAGTATGACCCGTCTTAAACTGAA
TCGAAGATTAGATAAGGTTCAGCGTGGTAATTGGGGCGATGTAAAGCATCTTCAAGAAGGTGTTTGGGAAATGCGAGAGT
TCTTCGGACCAGGTTGGCGCATGTATTACATCAAACATGGTGATGTAATCATTGTGATGCTTGGAGGTGGTGATAAATCT
ACACAGCAAAAGGATATAGAACGAGCTGTTGATTTATCAAAATCTTTAGAGGATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT171694 WP_000729704.1 NZ_CP060950:c3013589-3013329 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171694 NZ_CP078030:c2775-2485 [Acinetobacter variabilis]
ATGGTTAAAATAGCTGATCTACCTAGCTTTGACATGGCAGAAGCACTTAAGAGTGAAGAAGATATTGTAATGTATCTTAA
TATGGTTCTTGAAGAGAATGACCCTGCTGAACTAGCTCATGCACTTGGTGTAATTGCTAAAGCTCGTGGTATGACTCAAA
TCGCTAAAGAAGCAGGAATTGGACGTGAAGCACTTTACAAAGCACTCCGTCAAGATTCAGCACCTCGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGTCTAAAACTAACTGTGCAGAATGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References