Detailed information of TA system TA171671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4353071..4353750
• Replicon: chromosome
• Accession: NZ_CP060946
• Organism: Escherichia coli O157:H7 str. EC10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XN95
• Locus tag: H9197_RS20950
• Protein ID: WP_000057540.1
• Coordinates: 4353071..4353373 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: H9197_RS20955
• Protein ID: WP_000806442.1
• Coordinates: 4353409..4353750 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9197_RS20930 (4348334)	4348334..4349554	-	1221	WP_001251608.1	fosmidomycin MFS transporter	-
H9197_RS20935 (4349772)	4349772..4351424	+	1653	WP_000771768.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
H9197_RS20940 (4351461)	4351461..4351940	-	480	WP_000186631.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
H9197_RS20945 (4352144)	4352144..4352938	-	795	WP_000365180.1	TraB/GumN family protein	-
H9197_RS20950 (4353071)	4353071..4353373	+	303	WP_000057540.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9197_RS20955 (4353409)	4353409..4353750	+	342	WP_000806442.1	HigA family addiction module antitoxin	Antitoxin
H9197_RS20960 (4353808)	4353808..4356312	-	2505	WP_023155691.1	copper-exporting P-type ATPase CopA	-
H9197_RS20965 (4356574)	4356574..4357506	+	933	WP_023155692.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11781.35 Da
• Isoelectric Point: 10.2638

>T171671 WP_000057540.1 NZ_CP060946:4353071-4353373 [Escherichia coli O157:H7 str. EC10]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T171671 NZ_CP078015:c1552448-1551585 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GATTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT171671 WP_000806442.1 NZ_CP060946:4353409-4353750 [Escherichia coli O157:H7 str. EC10]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT171671 NZ_CP078015:c1552722-1552450 [Enterococcus faecalis]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: U9XN95

Antitoxin

• PDB: 2EBY
• AlphaFold DB: S1QAY3