Detailed information of TA system TA171616

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107476..107902
• Replicon: plasmid pEC12-2
• Accession: NZ_CP060934
• Organism: Escherichia coli strain EC12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H9199_RS25050
• Protein ID: WP_001372321.1
• Coordinates: 107476..107601 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107678..107902 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9199_RS25025 (102981)	102981..103802	-	822	WP_001234469.1	DUF932 domain-containing protein	-
H9199_RS25030 (103924)	103924..104211	-	288	WP_000107535.1	hypothetical protein	-
H9199_RS25035 (104236)	104236..104442	-	207	WP_000547939.1	hypothetical protein	-
H9199_RS25040 (104512)	104512..104685	+	174	Protein_109	hypothetical protein	-
H9199_RS25045 (105414)	105414..107081	+	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
H9199_RS25050 (107476)	107476..107601	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H9199_RS25055 (107543)	107543..107692	-	150	Protein_112	plasmid maintenance protein Mok	-
- (107680)	107680..107745	+	66	NuclAT_0	-	-
- (107680)	107680..107745	+	66	NuclAT_0	-	-
- (107680)	107680..107745	-	66	NuclAT_1	-	-
- (107678)	107678..107902	+	225	NuclAT_0	-	-
- (107678)	107678..107902	-	225	NuclAT_0	-	Antitoxin
- (107678)	107678..107902	-	225	NuclAT_0	-	Antitoxin
- (107678)	107678..107902	-	225	NuclAT_0	-	Antitoxin
- (107678)	107678..107902	-	225	NuclAT_0	-	Antitoxin
H9199_RS25060 (107714)	107714..107902	+	189	WP_001299721.1	hypothetical protein	-
H9199_RS25065 (107871)	107871..108633	-	763	Protein_114	plasmid SOS inhibition protein A	-
H9199_RS25070 (108630)	108630..109064	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
H9199_RS25075 (109119)	109119..111077	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
H9199_RS25080 (111136)	111136..111369	-	234	WP_000006004.1	DUF905 domain-containing protein	-
H9199_RS25085 (111425)	111425..111952	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
H9199_RS25090 (112254)	112254..112427	+	174	Protein_119	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T171616 WP_001372321.1 NZ_CP060934:c107601-107476 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T171616 NZ_CP077999:c176383-176102 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT171616 NZ_CP060934:c107902-107678 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin