Detailed information of TA system TA171549

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95393..95647
• Replicon: plasmid pEC14-2
• Accession: NZ_CP060925
• Organism: Escherichia coli strain EC14

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9201_RS23900
• Protein ID: WP_001312851.1
• Coordinates: 95393..95542 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	95586..95647 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9201_RS23860 (90945)	90945..91346	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H9201_RS23865 (91279)	91279..91536	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H9201_RS23870 (91629)	91629..92282	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H9201_RS23875 (93221)	93221..94078	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H9201_RS23880 (94071)	94071..94553	-	483	WP_001273588.1	hypothetical protein	-
H9201_RS23885 (94546)	94546..94593	-	48	WP_229471593.1	hypothetical protein	-
H9201_RS23890 (94584)	94584..94835	+	252	WP_223195197.1	replication protein RepA	-
H9201_RS23895 (94852)	94852..95109	-	258	WP_000083845.1	replication regulatory protein RepA	-
H9201_RS23900 (95393)	95393..95542	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (95586)	95586..95647	+	62	NuclAT_1	-	Antitoxin
- (95586)	95586..95647	+	62	NuclAT_1	-	Antitoxin
- (95586)	95586..95647	+	62	NuclAT_1	-	Antitoxin
- (95586)	95586..95647	+	62	NuclAT_1	-	Antitoxin
H9201_RS23905 (95903)	95903..95977	-	75	Protein_116	endonuclease	-
H9201_RS23910 (96223)	96223..96435	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H9201_RS23915 (96571)	96571..97131	-	561	WP_000139315.1	fertility inhibition protein FinO	-
H9201_RS23920 (97234)	97234..98094	-	861	WP_000704513.1	alpha/beta hydrolase	-
H9201_RS23925 (98153)	98153..98899	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..159110	159110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171549 WP_001312851.1 NZ_CP060925:c95542-95393 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171549 NZ_CP077969:c2007502-2007248 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT171549 NZ_CP060925:95586-95647 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin