Detailed information of TA system TA171353

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1903439..1904274
• Replicon: chromosome
• Accession: NZ_CP060892
• Organism: Escherichia coli strain EC22

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: H9207_RS09100
• Protein ID: WP_000854761.1
• Coordinates: 1903439..1903816 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: H9207_RS09105
• Protein ID: WP_001295723.1
• Coordinates: 1903906..1904274 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9207_RS09060 (1898828)	1898828..1899094	-	267	WP_087757644.1	EutP/PduV family microcompartment system protein	-
H9207_RS09065 (1899464)	1899464..1899853	-	390	WP_001445118.1	IS110 family transposase	-
H9207_RS09070 (1900042)	1900042..1900266	-	225	Protein_1788	transposase	-
H9207_RS09075 (1900347)	1900347..1900751	+	405	WP_000839179.1	transposase	-
H9207_RS09080 (1900748)	1900748..1901095	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
H9207_RS09085 (1901144)	1901144..1902683	+	1540	Protein_1791	IS66-like element ISEc22 family transposase	-
H9207_RS09090 (1903114)	1903114..1903194	-	81	Protein_1792	hypothetical protein	-
H9207_RS09095 (1903294)	1903294..1903442	-	149	Protein_1793	DUF5983 family protein	-
H9207_RS09100 (1903439)	1903439..1903816	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
H9207_RS09105 (1903906)	1903906..1904274	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9207_RS09110 (1904437)	1904437..1904658	-	222	WP_000692341.1	DUF987 domain-containing protein	-
H9207_RS09115 (1904721)	1904721..1905197	-	477	WP_001186774.1	RadC family protein	-
H9207_RS09120 (1905213)	1905213..1905686	-	474	WP_000855059.1	antirestriction protein	-
H9207_RS09125 (1906028)	1906028..1906846	-	819	WP_001234729.1	DUF932 domain-containing protein	-
H9207_RS09130 (1906964)	1906964..1907159	-	196	Protein_1800	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T171353 WP_000854761.1 NZ_CP060892:c1903816-1903439 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T171353 NZ_CP077897:c378924-378607 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT171353 WP_001295723.1 NZ_CP060892:c1904274-1903906 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT171353 NZ_CP077897:c379136-378927 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATAGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52