Detailed information of TA system TA171311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1781..2328
• Replicon: plasmid pEC26-2
• Accession: NZ_CP060881
• Organism: Escherichia coli strain EC26

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: H9209_RS23550
• Protein ID: WP_013009346.1
• Coordinates: 2050..2328 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E0WH52
• Locus tag: H9209_RS23545
• Protein ID: WP_000079932.1
• Coordinates: 1781..2050 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9209_RS23535 (H9209_23295)	1..873	+	873	WP_000729438.1	incFII family plasmid replication initiator RepA	-
H9209_RS23540 (H9209_23300)	1568..1726	+	159	WP_000829588.1	hypothetical protein	-
H9209_RS23545 (H9209_23305)	1781..2050	+	270	WP_000079932.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
H9209_RS23550 (H9209_23310)	2050..2328	+	279	WP_013009346.1	type II toxin-antitoxin system toxin YacB	Toxin
H9209_RS23555 (H9209_23315)	2524..2820	-	297	WP_001419651.1	hypothetical protein	-
H9209_RS23560 (H9209_23320)	3165..3752	+	588	WP_001418189.1	serine acetyltransferase	-
H9209_RS23565 (H9209_23325)	4034..4627	-	594	WP_241424678.1	ProQ/FINO family protein	-
H9209_RS23570 (H9209_23330)	4981..6327	+	1347	WP_001132021.1	protein YagA	-
H9209_RS23575 (H9209_23335)	6857..7159	-	303	WP_001245884.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83754	83754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10748.35 Da
• Isoelectric Point: 8.4615

>T171311 WP_013009346.1 NZ_CP060881:2050-2328 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
VLRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T171311 NZ_CP077885:c1867061-1866699 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10268.58 Da
• Isoelectric Point: 7.2055

>AT171311 WP_000079932.1 NZ_CP060881:1781-2050 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVSDK

Nucleotide

• Length: 270 bp

>AT171311 NZ_CP077885:c1867228-1867058 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5U8RN22