Detailed information of TA system TA171247

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104896..105317
• Replicon: plasmid pEC29-NDM-5
• Accession: NZ_CP060868
• Organism: Escherichia coli strain EC29

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H9211_RS24240
• Protein ID: WP_096937776.1
• Coordinates: 105192..105317 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	104896..105094 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9211_RS24200 (100588)	100588..101982	+	1395	WP_001037799.1	carbohydrate porin	-
H9211_RS24205 (102228)	102228..102332	-	105	Protein_113	thiosulfate reductase cytochrome B subunit	-
H9211_RS24210 (102389)	102389..103086	+	698	WP_241418171.1	IS1-like element IS1A family transposase	-
H9211_RS24215 (103213)	103213..103725	+	513	WP_223350513.1	potassium channel family protein	-
H9211_RS24220 (103810)	103810..104061	+	252	Protein_116	iron-containing alcohol dehydrogenase	-
H9211_RS24225 (104350)	104350..104667	-	318	Protein_117	IS110 family transposase	-
H9211_RS24230 (104822)	104822..104927	+	106	Protein_118	plasmid SOS inhibition protein A	-
- (104896)	104896..105094	+	199	NuclAT_0	-	Antitoxin
- (104896)	104896..105094	+	199	NuclAT_0	-	Antitoxin
- (104896)	104896..105094	+	199	NuclAT_0	-	Antitoxin
- (104896)	104896..105094	+	199	NuclAT_0	-	Antitoxin
H9211_RS24235 (105101)	105101..105250	+	150	Protein_119	DUF5431 family protein	-
H9211_RS24240 (105192)	105192..105317	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
H9211_RS24245 (105618)	105618..105915	-	298	Protein_121	hypothetical protein	-
H9211_RS24250 (105985)	105985..106191	+	207	WP_000275854.1	hypothetical protein	-
H9211_RS24255 (106216)	106216..106503	+	288	WP_000107242.1	hypothetical protein	-
H9211_RS24260 (106622)	106622..107443	+	822	WP_001234465.1	DUF932 domain-containing protein	-
H9211_RS24265 (107739)	107739..108311	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
H9211_RS24270 (108681)	108681..109064	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H9211_RS24275 (109255)	109255..109902	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
H9211_RS24280 (110038)	110038..110253	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A)	-	1..147459	147459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T171247 WP_096937776.1 NZ_CP060868:105192-105317 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T171247 NZ_CP077867:2351992-2352354 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT171247 NZ_CP060868:104896-105094 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin