Detailed information of TA system TA171061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2685349..2685914
• Replicon: chromosome
• Accession: NZ_CP060825
• Organism: Streptomyces sp. CRPJ-33

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: IAG43_RS11750
• Protein ID: WP_187740697.1
• Coordinates: 2685349..2685717 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: IAG43_RS11755
• Protein ID: WP_187740698.1
• Coordinates: 2685717..2685914 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAG43_RS11735	2681441..2682229	-	789	WP_187740694.1	hypothetical protein	-
IAG43_RS11740	2682295..2682936	-	642	WP_187740695.1	helix-turn-helix domain-containing protein	-
IAG43_RS11745	2683433..2685217	-	1785	WP_187740696.1	DEAD/DEAH box helicase	-
IAG43_RS11750	2685349..2685717	-	369	WP_187740697.1	Fic family protein	Toxin
IAG43_RS11755	2685717..2685914	-	198	WP_187740698.1	Arc family DNA-binding protein	Antitoxin
IAG43_RS11760	2685947..2687452	-	1506	WP_187740699.1	MFS transporter	-
IAG43_RS11770	2687786..2690113	-	2328	WP_187740700.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (12-80)

Antitoxin

• Arc-like DNA binding domain (5-33)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13723.67 Da
• Isoelectric Point: 5.5782

>T171061 WP_187740697.1 NZ_CP060825:c2685717-2685349 [Streptomyces sp. CRPJ-33]
MKYLTLPELLRLTERLGADEVRDYGLLDSALARPQSSVFGQDAYPDVWQKAAALMESLARNHGLVDGNKRIAWYATWVFL
HMNGHPLDAGFDVDEAERFVLGVCQGHLDVPKIAEQLPRFAK

Nucleotide

• Length: 369 bp

>T171061 NZ_CP077823:2352837-2353154 [Klebsiella africana]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7379.27 Da
• Isoelectric Point: 5.5977

>AT171061 WP_187740698.1 NZ_CP060825:c2685914-2685717 [Streptomyces sp. CRPJ-33]
MAGLNLRFTEEELDALRKRAEAEGRSMQSFAHDAVITAINEHSRLFNEAADHVLKVSEELNRRLA

Nucleotide

• Length: 198 bp

>AT171061 NZ_CP077823:2353230-2353526 [Klebsiella africana]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCCGAA
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA