Detailed information of TA system TA170891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 45413..46074
• Replicon: chromosome
• Accession: NZ_CP060737
• Organism: Enterobacter roggenkampii isolate enterobacter cloacae

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9W87_RS00230
• Protein ID: WP_023293716.1
• Coordinates: 45676..46074 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B3V1Y0
• Locus tag: H9W87_RS00225
• Protein ID: WP_045414808.1
• Coordinates: 45413..45679 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W87_RS00200	41466..42794	-	1329	WP_025910681.1	PTS sugar transporter subunit IIC	-
H9W87_RS00205	42806..43120	-	315	WP_021242703.1	PTS sugar transporter subunit IIB	-
H9W87_RS00210	43414..44364	+	951	WP_023293714.1	LacI family transcriptional regulator	-
H9W87_RS00215	44426..44722	-	297	WP_021242704.1	hypothetical protein	-
H9W87_RS00220	44864..45283	+	420	WP_032654363.1	GNAT family N-acetyltransferase	-
H9W87_RS00225	45413..45679	+	267	WP_045414808.1	virulence protein	Antitoxin
H9W87_RS00230	45676..46074	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9W87_RS00235	46214..47014	+	801	WP_047745055.1	lipoprotein NlpA	-
H9W87_RS00240	47138..47917	+	780	WP_047745054.1	MBL fold metallo-hydrolase	-
H9W87_RS00245	47944..48897	+	954	WP_045414812.1	helix-turn-helix domain-containing protein	-
H9W87_RS00250	49080..50777	+	1698	WP_187798040.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T170891 WP_023293716.1 NZ_CP060737:45676-46074 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T170891 NZ_CP077763:3949123-3949506 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9981.32 Da
• Isoelectric Point: 4.6310

>AT170891 WP_045414808.1 NZ_CP060737:45413-45679 [Enterobacter roggenkampii]
MERTAKLFKKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT170891 NZ_CP077763:3948881-3949123 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A8B3V1Y0