Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2060786..2061617 Replicon chromosome
Accession NZ_CP060708
Organism Escherichia coli strain MFDpir

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag H9446_RS10185 Protein ID WP_000854814.1
Coordinates 2061243..2061617 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag H9446_RS10180 Protein ID WP_001285584.1
Coordinates 2060786..2061154 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9446_RS10165 2058453..2059985 + 1533 WP_001350525.1 hypothetical protein -
H9446_RS10170 2059982..2060428 + 447 WP_000187523.1 RadC family protein -
H9446_RS10175 2060491..2060712 + 222 WP_000692323.1 DUF987 domain-containing protein -
H9446_RS10180 2060786..2061154 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
H9446_RS10185 2061243..2061617 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
H9446_RS10190 2061614..2061808 + 195 WP_000988600.1 hypothetical protein -
H9446_RS10195 2061821..2061934 + 114 WP_001161660.1 DUF957 domain-containing protein -
H9446_RS10200 2062223..2062351 - 129 Protein_1990 transposase domain-containing protein -
H9446_RS10205 2062423..2062605 + 183 WP_001016348.1 hypothetical protein -
H9446_RS10210 2062706..2063035 - 330 WP_000450409.1 DUF496 family protein -
H9446_RS10215 2063207..2064265 - 1059 WP_001200891.1 FUSC family protein -
H9446_RS10220 2064463..2064936 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
H9446_RS10225 2065055..2066221 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T170789 WP_000854814.1 NZ_CP060708:2061243-2061617 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T170789 NZ_CP077704:c4214959-4214675 [Salmonella enterica subsp. enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGATGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT170789 WP_001285584.1 NZ_CP060708:2060786-2061154 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT170789 NZ_CP077704:c4215191-4214949 [Salmonella enterica subsp. enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References