Detailed information of TA system TA170264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 183641..184146
• Replicon: chromosome
• Accession: NZ_CP060392
• Organism: Pseudomonas aeruginosa strain 1903031130

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: H8A93_RS00800
• Protein ID: WP_003083773.1
• Coordinates: 183641..183922 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: H8A93_RS00805
• Protein ID: WP_003083775.1
• Coordinates: 183919..184146 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8A93_RS00775	178892..180241	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
H8A93_RS00780	180290..180976	+	687	WP_003083762.1	FadR family transcriptional regulator	-
H8A93_RS00785	181077..181811	+	735	WP_003083764.1	GntR family transcriptional regulator	-
H8A93_RS00790	182015..182401	+	387	WP_003083767.1	aegerolysin family protein	-
H8A93_RS00795	182433..183341	-	909	WP_003083769.1	LysR family transcriptional regulator	-
H8A93_RS00800	183641..183922	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
H8A93_RS00805	183919..184146	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
H8A93_RS00810	184322..184942	-	621	WP_003101226.1	hypothetical protein	-
H8A93_RS00815	185043..185543	+	501	WP_003083778.1	LEA type 2 family protein	-
H8A93_RS00820	185616..185957	+	342	WP_014603467.1	alkylphosphonate utilization protein	-
H8A93_RS00825	186039..187466	-	1428	WP_003083784.1	GABA permease	-
H8A93_RS00830	187635..189128	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T170264 WP_003083773.1 NZ_CP060392:c183922-183641 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T170264 NZ_CP077242:c919552-919238 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT170264 WP_003083775.1 NZ_CP060392:c184146-183919 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT170264 NZ_CP077242:c919788-919555 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9