Detailed information of TA system TA170248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 7244314..7246059
• Replicon: chromosome
• Accession: NZ_CP060295
• Organism: Variovorax sp. PAMC26660

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H7F35_RS33995
• Protein ID: WP_187110857.1
• Coordinates: 7245187..7246059 (-)
• Length: 291 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: H7F35_RS33990
• Protein ID: WP_187110856.1
• Coordinates: 7244314..7245147 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7F35_RS33970	7239477..7240955	-	1479	WP_187110854.1	aldehyde dehydrogenase	-
H7F35_RS33975	7240967..7241755	-	789	WP_187114509.1	aspartate dehydrogenase	-
H7F35_RS33980	7241767..7243479	-	1713	WP_187110855.1	thiamine pyrophosphate-binding protein	-
H7F35_RS33985	7243472..7244302	-	831	WP_187114510.1	SDR family oxidoreductase	-
H7F35_RS33990	7244314..7245147	-	834	WP_187110856.1	IclR family transcriptional regulator	Antitoxin
H7F35_RS33995	7245187..7246059	-	873	WP_187110857.1	alpha/beta fold hydrolase	Toxin
H7F35_RS34000	7246067..7246618	-	552	WP_187110858.1	cupin domain-containing protein	-
H7F35_RS34005	7246664..7247479	-	816	WP_187110859.1	SDR family oxidoreductase	-
H7F35_RS34010	7247487..7248473	-	987	WP_187110860.1	tripartite tricarboxylate transporter substrate binding protein	-
H7F35_RS34015	7248658..7249917	-	1260	WP_187110861.1	D-amino acid dehydrogenase	-
H7F35_RS34020	7249968..7250984	-	1017	WP_187110862.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (48-282)

Antitoxin

• Transcription regulator IclR, N-terminal (19-68)

Sequences

Toxin

Protein

• Length: 291 a.a.
• Molecular weight: 29723.96 Da
• Isoelectric Point: 6.5955

>T170248 WP_187110857.1 NZ_CP060295:c7246059-7245187 [Variovorax sp. PAMC26660]
MSDATATDAQTLPASELALLDARFPARTVPVGGGTAVSVREHGAGPALVCLHGIGSGAASWLHVALLLAPQARVIAWDAP
GYGASTPLASATPTAVDYAERLHALLDALDVQSCVLVGHSLGALTAASAARNGSALASRIRRLVLISPAAGYGAPSRVEA
RAKVHTERLASLDELGIAGMAAKRSGRLVSEGASELARQWVRWNMARLNDGGYRQAVELLCNGNLLADLPPAMPVRVACG
TLDVVTTPDACAEVARQCGVTLEPIEGAGHASYVEQPQAVAALLREALAA

Nucleotide

• Length: 873 bp

>T170248 NZ_CP077236:c1071691-1071371 [Lelliottia amnigena]
ATGCAAACCTCAACTGTACCGGTCACGGTGCCGGTTTTGTCACGCCTGTCGCCCGTGCAGGTCTGGCAGCAACTGTTAAC
GTACCTGTTAGAACACCATTACGGTCTGACACTTAACGACACGCCATTCCATGATGACGCGGCCATCCAGGAGCATATCG
AAGCGGGAATAACATTGGCTGATGCGGTGAATTTCCTGGTGGAACGTTATGAGCTGGTGCGCACCGACCGCAAAGGATTT
ACCTGGCAGGAGCAGACGCCATTCCTGACCGCTACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAACACGTA
A

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30606.99 Da
• Isoelectric Point: 5.4819

>AT170248 WP_187110856.1 NZ_CP060295:c7245147-7244314 [Variovorax sp. PAMC26660]
MANDNEVMEEGADRYNVPALERGLRVLCEFSRESRTLSAPELARRFDLPRSTVFRLLTTLENMGFLERAEGGRDYRLGLA
VLRLGFEYLASLELTQLGTPLLNRLCDELRTPCNLVVRDGRSIVYVAKVAPPTPFASSVTVGTRLPAHATVLGRVLLEDL
TLPQLRALYPEDKLETFSPSTPKTVNELFDMVQADRSRGYVLGEGFFESNISSIAAPVRDHSGHIVAALGATITSGQIEE
NRMDEMVLRVRSTAEEISGLLNYSPARANKVVPLRSA

Nucleotide

• Length: 834 bp

>AT170248 NZ_CP077236:c1072093-1071758 [Lelliottia amnigena]
ATGAATAAAGACGTTACACAAACCTGGGGTCTTAAACGAGAGATCACACCGTGTTTTGGAGCACGGCTGGTGCAGGAAGG
CCATCGTCTGCATTTTCTGGCGGACTGGGCAGGATTCACCGGTATGTTCAGTGAGGTACAGGCCCTACAACTGGATGAAG
CCTTCCCTCATTTTGTCGCACATCTGGAGCTGATGCTGCTCTCCTGTGAACTGAATCCCCGGTACGCGCACTGCGTCACG
CTGTACCGTAACGGGTTGACCTGTGAAGCCGATACGCTGGGTAGTCATGGGTTTGTATATATCGCAATTTATCCCTCAAA
ACAGGTTAAAGACTGA