Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5337327..5337952 Replicon chromosome
Accession NZ_CP060281
Organism Klebsiella pneumoniae strain ent1077

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H7U30_RS25685 Protein ID WP_002882817.1
Coordinates 5337327..5337710 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H7U30_RS25690 Protein ID WP_004150355.1
Coordinates 5337710..5337952 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7U30_RS25670 5334693..5335595 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H7U30_RS25675 5335592..5336227 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H7U30_RS25680 5336224..5337153 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H7U30_RS25685 5337327..5337710 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H7U30_RS25690 5337710..5337952 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H7U30_RS25695 5338157..5339074 + 918 WP_029884108.1 alpha/beta hydrolase -
H7U30_RS25700 5339088..5340029 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H7U30_RS25705 5340074..5340511 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H7U30_RS25710 5340508..5341368 - 861 WP_002882807.1 virulence factor BrkB family protein -
H7U30_RS25715 5341362..5341961 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T170226 WP_002882817.1 NZ_CP060281:c5337710-5337327 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T170226 NZ_CP077211:c924580-924395 [Enterobacter cloacae]
GTGAAAAGTGCGGATGTCATTACTGTTCTGGTTAGGCATGGCTGGAAATGTGTAAGAACGAAAGGAAGCCATCATCAGTT
TCGCCACCCAGTACATGCGGGGTTGGTGACCGTTCCACATCCCAAAAAGGACATAAAACCCGGCACCCTCGCGCAAATCT
GGCGGCAGGCGGGAATCAAACACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT170226 WP_004150355.1 NZ_CP060281:c5337952-5337710 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT170226 NZ_CP077211:c924380-923976 [Enterobacter cloacae]
ATGTTTTATCCGGCTTACATTCATTCTGATCCCGACGGTAGCGCCAGCGGTTTTTTTCCTGACGTTCCTGGTTGTTTTTT
TGCCGGCAATTCTCTGGATGATGCTTTTCAGGATGCACGTGATGCACTGACAGCTCACTTCGAAGCGCTATTTGAAATGG
ATGAAGAGTTACCTCTCCCTGGCAATGTTGAAGCACATCTTGAAGCCACACCGGGTGATTTTATCGGCGGACAATGGTTG
CTTGTAGATATCAATATGAAGCAATTCGACGGTAAGGCCGAGCGTATCAATATCACCCTGCCTCGTCGATTGCTGGTGAA
AATTGATTCTTTTGTGAATGAGCATCCGCAGTTTAGTAACCGAAGCGCTTTTCTTGCTGAGGCAGCGCGTCGCGTGCTGC
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References