Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4117532..4118151 Replicon chromosome
Accession NZ_CP060281
Organism Klebsiella pneumoniae strain ent1077

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H7U30_RS19930 Protein ID WP_002892050.1
Coordinates 4117933..4118151 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag H7U30_RS19925 Protein ID WP_002892066.1
Coordinates 4117532..4117906 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7U30_RS19915 4112684..4113877 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7U30_RS19920 4113900..4117046 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
H7U30_RS19925 4117532..4117906 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
H7U30_RS19930 4117933..4118151 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
H7U30_RS19935 4118310..4118876 + 567 WP_002892030.1 maltose O-acetyltransferase -
H7U30_RS19940 4118848..4118988 - 141 WP_004147370.1 hypothetical protein -
H7U30_RS19945 4119009..4119479 + 471 WP_002892026.1 YlaC family protein -
H7U30_RS19950 4119486..4120943 - 1458 WP_029883993.1 PLP-dependent aminotransferase family protein -
H7U30_RS19955 4121044..4121742 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
H7U30_RS19960 4121739..4121879 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
H7U30_RS19965 4121879..4122142 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4122202..4123248 1046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T170223 WP_002892050.1 NZ_CP060281:4117933-4118151 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T170223 NZ_CP077211:c671999-671517 [Enterobacter cloacae]
ATGGAAATAAATGTCACTGCGCCTGCTTTACTGACAGAGGAGCATGTTCTTCATTCGTTCGATTGCGGGAATGCGGTACT
TGATGACTGGCTTCGCCGGCGTGCGTTGAAGAATCAGCATCTTAATGCTTCCCGGACATTCGTCATCTGTCCTGAAGGTA
CTGCGTGTGTAGTGGGTTATTATTCCCTTGCGACGGGCTCAGTTAGTCATGTCGACTTGAGTCGTAGTCTGCGACAGAAT
ATGCCCGATCCCGTACCGGTTGTTTTGCTGGGCCGCCTTGCAGTCGATGTATCCACTCAGGGCAATAACTTCGGGAAATG
GTTGCTGAATGATGCTGTGATGCGCGTATCGAATCTGGCGGATCAGGTCGGCATCAAGGCTATCATGGTGCATGCAATTG
ATGAGCGGGCTAAAGCGTTCTATGAATACTTTGGTTTTGTGCAGTCACCGGTTGCGGCTAACACGCTTTTCTACAAAATA
TAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT170223 WP_002892066.1 NZ_CP060281:4117532-4117906 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT170223 NZ_CP077211:c672274-671990 [Enterobacter cloacae]
ATGCAAACTCCAGTCCGTAAATCCGTCCGTGACAAGCAAATCAATATCCGCGCCACGGATGAAGAACGTGCGGTGATTGA
TTACGCTGCGAGTCTGGTTAATAAAAACCGTACTGATTTCATCATTGAAAGGGCTGTGCATGAGGCCCAGAACATCATTC
TGGACCAGCGTGTGTTCGTTCTTGATGATGCCCGTTACCAGGCGTTCATCAGGCAGCTTGAAGCCCCTGTGCAGAATGTT
GAAGGTCGTCAGCGACTGATGGATGTAAAACCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References