Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 201331..202070 Replicon chromosome
Accession NZ_CP060281
Organism Klebsiella pneumoniae strain ent1077

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag H7U30_RS00980 Protein ID WP_004901283.1
Coordinates 201585..202070 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H7U30_RS00975 Protein ID WP_003026799.1
Coordinates 201331..201597 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7U30_RS00960 196835..198904 + 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -
H7U30_RS00965 199200..200569 + 1370 WP_087661561.1 IS3 family transposase -
H7U30_RS00970 200770..201198 + 429 WP_004901287.1 GFA family protein -
H7U30_RS00975 201331..201597 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H7U30_RS00980 201585..202070 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
H7U30_RS00985 202395..203441 - 1047 WP_022615678.1 IS481-like element ISKpn28 family transposase -
H7U30_RS00990 203515..203667 + 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
H7U30_RS00995 203969..205588 + 1620 WP_029884695.1 ATP-binding cassette domain-containing protein -
H7U30_RS01000 205687..205899 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
H7U30_RS01005 206151..206441 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 199844..203441 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T170210 WP_004901283.1 NZ_CP060281:201585-202070 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T170210 NZ_CP077198:627275-627676 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCTGGAATGAAAGG
GGCAGTTGATATTCCTAAGCTTCAAGGTGCACTAGGTCGAATCGATAATGCTATCGTTTATGAAGGACTGGATGATGTTT
TCGAAATCGCAGCTAAATATACTGCTTGTATAGCGGTTTCACATGCGCTACCAGATGCCAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAATGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCGCAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT170210 WP_003026799.1 NZ_CP060281:201331-201597 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT170210 NZ_CP077198:627105-627275 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGCAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAAACATTCACTAAATTGG
CTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References