Detailed information of TA system TA170100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5668090..5668744
• Replicon: chromosome
• Accession: NZ_CP060201
• Organism: Pseudomonas protegens strain H1F5C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7G8YM43
• Locus tag: GGI48_RS26250
• Protein ID: WP_016965723.1
• Coordinates: 5668394..5668744 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GGI48_RS26245
• Protein ID: WP_179600748.1
• Coordinates: 5668090..5668407 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGI48_RS26225	5663393..5664145	-	753	WP_016965728.1	UDP-2,3-diacylglucosamine diphosphatase	-
GGI48_RS26230	5664142..5664645	-	504	WP_016965727.1	peptidyl-prolyl cis-trans isomerase	-
GGI48_RS26235	5664916..5666616	+	1701	WP_179600746.1	glutamine--tRNA ligase/YqeY domain fusion protein	-
GGI48_RS26240	5666635..5668017	+	1383	WP_016965725.1	cysteine--tRNA ligase	-
GGI48_RS26245	5668090..5668407	-	318	WP_179600748.1	transcriptional regulator	Antitoxin
GGI48_RS26250	5668394..5668744	-	351	WP_016965723.1	hypothetical protein	Toxin
GGI48_RS26255	5668983..5670446	-	1464	WP_015636299.1	NADH-quinone oxidoreductase subunit NuoN	-
GGI48_RS26260	5670454..5671986	-	1533	WP_016965722.1	NADH-quinone oxidoreductase subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-101)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13980.08 Da
• Isoelectric Point: 8.9805

>T170100 WP_016965723.1 NZ_CP060201:c5668744-5668394 [Pseudomonas protegens]
MNALFIELPAFARYRRDFLDDELFCELQIELLRHPHAGVLIQGTGGLRKMRFVDERRNKGKRGGLRVIYYWWSGGAQFWL
FTLFDKEQMDDLLPQQRQQLKQLLEREIEARRHDQT

Nucleotide

• Length: 351 bp

>T170100 NZ_CP077082:c3888313-3887948 [Pseudomonas sp. OE 28.3]
ATGGATTGGGACGTTGAATACACGGATGAATTCGCAGAATGGTGGGATGAACTGGGCGCAAAGGAGCAAGTCTCCGTGTC
CGCCAGCGTAGATCTGCTGGGGCTTTTCGGGCCTGGCCTGGGCTTCCCCCATACCAGTGATATCAAGGGTTCCCGGCATG
GCAACCTGCGGGAACTGCGGGTCCGGCACGCCGGCCGGCCCTATCGAGTGCTGTATGCATTTGATCCCCGGCGCTGCGCT
GTCCTGCTGATCGGCGGCGATAAAGCCGCACACCCCAGGTGGTACGAGGTGCATGTGCCCTTGGCCGAAAATGTGTACGA
CATCCATTTGCAAACATTGCGTAAAGAGGCCCGTCACCATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12038.87 Da
• Isoelectric Point: 10.4439

>AT170100 WP_179600748.1 NZ_CP060201:c5668407-5668090 [Pseudomonas protegens]
MTKRDIFSELVEGIDALTQERQGKITLRSHKVKLDKLPPITAAEVMNIRQQLNLSRSVFAMYLRTNTRTLENWEQGRATP
NAQATTLIRLVERYPDTVERLASLA

Nucleotide

• Length: 318 bp

>AT170100 NZ_CP077082:c3887955-3887650 [Pseudomonas sp. OE 28.3]
ATGGCTAAAAAATTCGCTGAACTTCAAGCGCGCATGACCCCGCAGTCGCGTGCCGAAGCCGAGCAGCTGTTCCGCCAGCA
CCTCAAGGAAATGCCCCTGCATGAGCTGCGCAAGGCCCAGCAACTGAGCCAGGAAAGCCTGGCCAAGGCGTTGAATATCA
ACCAGGCAGCGGTTTCCAAGATGGAGCGGCGTACCGACATGTACATCAGCACGCTGCGTGACTACATCCGGGCAATGGGC
GGCGAGTTGGAAATCATCGCGACCTTCCCGGATGGCCAGGTCAAGATCGACAACTTCGCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A7G8YM43