Detailed information of TA system TA170096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 911146..911765
• Replicon: chromosome
• Accession: NZ_CP060201
• Organism: Pseudomonas protegens strain H1F5C

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2J7U9Z9
• Locus tag: GGI48_RS04170
• Protein ID: WP_041120604.1
• Coordinates: 911146..911328 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GGI48_RS04175
• Protein ID: WP_179597148.1
• Coordinates: 911364..911765 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGI48_RS04145	906702..907940	-	1239	WP_179597144.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
GGI48_RS04150	907961..908479	-	519	WP_080966932.1	sugar O-acetyltransferase	-
GGI48_RS04155	909015..909589	+	575	Protein_830	hypothetical protein	-
GGI48_RS04160	909743..910261	+	519	WP_179597146.1	hypothetical protein	-
GGI48_RS04165	910347..910553	+	207	Protein_832	hypothetical protein	-
GGI48_RS04170	911146..911328	+	183	WP_041120604.1	type II toxin-antitoxin system HicA family toxin	Toxin
GGI48_RS04175	911364..911765	+	402	WP_179597148.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GGI48_RS04180	911923..913326	-	1404	WP_179597150.1	PLP-dependent aminotransferase family protein	-
GGI48_RS04185	913414..913860	+	447	WP_179597152.1	GNAT family N-acetyltransferase	-
GGI48_RS04190	913857..914477	+	621	WP_179597154.1	NAD(P)H-dependent oxidoreductase	-
GGI48_RS04195	914505..914936	+	432	WP_179597156.1	carboxymuconolactone decarboxylase family protein	-
GGI48_RS04200	915510..915632	-	123	Protein_839	PAAR domain-containing protein	-
GGI48_RS04205	915686..916117	-	432	WP_179597158.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	893902..917730	23828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6885.09 Da
• Isoelectric Point: 11.2684

>T170096 WP_041120604.1 NZ_CP060201:911146-911328 [Pseudomonas protegens]
VQSRQLIKELEAAGWVLERVTGSHHLFKHPYRPNTVPVPHPKKDLPRGTVRAIRKLAELI

Nucleotide

• Length: 183 bp

>T170096 NZ_CP077082:c1818879-1818571 [Pseudomonas sp. OE 28.3]
ATGACTCAAACACTGACGACATTTGATATGGCCGAGCTGCTCGACAGTGATGAGGCCATCAGCGAATACCTTTCTCAGGT
GCTGGCTGACGGTGACAACGAAGAGTTCCTTCGCGCCGTAGGCTACGTAGCCAAAGCCCGTGGCATGACAAAGATCGCCA
CGGATGCGGGCGTAGGGCGCGAGAGCTTGTACAAGGCATTTGCCCCGGGCGCAAAACCTCGTTTTGAAACGGTTCTGAAG
GTGCTTCACTCACTGGGCATTGATCTGTTGGCTCGGCCTGGGCATACCAGCAATTATTCGATTGTTTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14644.66 Da
• Isoelectric Point: 5.1754

>AT170096 WP_179597148.1 NZ_CP060201:911364-911765 [Pseudomonas protegens]
VQYPICIEWGDEHTATGIQIPDIPGAVTAGDSFESAYSAAIEIAHVMLEELARAGHAIPLPSPTRKHRANPDFEGMGWGM
LEIDITPYLGKTEKVNVTLPGYVIQQIDRFVREHNIKSRSSFLADAAMEKLGR

Nucleotide

• Length: 402 bp

>AT170096 NZ_CP077082:c1819175-1818882 [Pseudomonas sp. OE 28.3]
GTGCGTTATTCGATCCAGCAAACCCAAGCCTTCACTCAATGGTGCTCGGCAATTCGCGATCTGCGAGCAAAAATCACCAT
TGCTCGACGTATCGAGCGAGCCAGTGTGGGCAATCTGGGGGATTTCAAGGTGCTGGGCGGCGGTGTTTCAGAGATGCGGA
TAGACGTAGGTGCAGGTTATCGCGTTTATTTCACCCTGCGCGGCAGGGAGGTTGTCATCTTGCTGGCAGGTGGCGACAAG
TCCTCACAAGAGGCCGATATCCGGCGAGCAAAAAAATTAGCAAAGGAGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A2J7U9Z9