Detailed information of TA system TA169939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1378921..1379591
• Replicon: chromosome
• Accession: NZ_CP060086
• Organism: Pseudomonas aeruginosa strain JNQH-PA57

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5409_RS06450
• Protein ID: WP_033868686.1
• Coordinates: 1379172..1379591 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H5409_RS06445
• Protein ID: WP_033868685.1
• Coordinates: 1378921..1379175 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5409_RS06425	1374855..1375862	-	1008	WP_033868682.1	hypothetical protein	-
H5409_RS06430	1375948..1376172	+	225	WP_009363662.1	AlpA family phage regulatory protein	-
H5409_RS06435	1376175..1377053	+	879	WP_033868683.1	helicase RepA family protein	-
H5409_RS06440	1377040..1377792	+	753	WP_033868684.1	hypothetical protein	-
H5409_RS06445	1378921..1379175	+	255	WP_033868685.1	Arc family DNA-binding protein	Antitoxin
H5409_RS06450	1379172..1379591	+	420	WP_033868686.1	type II toxin-antitoxin system VapC family toxin	Toxin
H5409_RS06455	1379748..1380518	+	771	WP_033868687.1	P-type conjugative transfer protein TrbJ	-
H5409_RS06460	1380529..1380687	+	159	WP_153570825.1	hypothetical protein	-
H5409_RS06465	1380689..1382161	+	1473	WP_121382693.1	P-type conjugative transfer protein TrbL	-
H5409_RS06470	1382230..1382472	+	243	WP_003137888.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
H5409_RS06475	1382484..1382768	+	285	WP_003137890.1	hypothetical protein	-
H5409_RS06480	1382771..1383139	+	369	WP_033868688.1	conjugal transfer transcriptional regulator TraJ	-
H5409_RS06485	1383178..1383636	-	459	WP_065426345.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1374855..1395529	20674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14883.08 Da
• Isoelectric Point: 5.1890

>T169939 WP_033868686.1 NZ_CP060086:1379172-1379591 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYVSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T169939 NZ_CP076802:c43104-42745 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGTCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTATGGAGATAAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.81 Da
• Isoelectric Point: 6.4760

>AT169939 WP_033868685.1 NZ_CP060086:1378921-1379175 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKAPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT169939 NZ_CP076802:c42745-42446 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA