Detailed information of TA system TA169851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2503687..2504325
• Replicon: chromosome
• Accession: NZ_CP060065
• Organism: Escherichia coli strain G5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: H4P49_RS12155
• Protein ID: WP_000813794.1
• Coordinates: 2504149..2504325 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H4P49_RS12150
• Protein ID: WP_001270286.1
• Coordinates: 2503687..2504103 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P49_RS12130	2498839..2499780	-	942	WP_001251304.1	ABC transporter permease	-
H4P49_RS12135	2499781..2500794	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H4P49_RS12140	2500812..2501957	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H4P49_RS12145	2502202..2503608	-	1407	WP_047601218.1	PLP-dependent aminotransferase family protein	-
H4P49_RS12150	2503687..2504103	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H4P49_RS12155	2504149..2504325	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H4P49_RS12160	2504547..2504777	+	231	WP_000494244.1	YncJ family protein	-
H4P49_RS12165	2504869..2506830	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H4P49_RS12170	2506903..2507439	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H4P49_RS12175	2507492..2508706	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T169851 WP_000813794.1 NZ_CP060065:c2504325-2504149 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T169851 NZ_CP076704:c4204459-4204169 [Escherichia coli O8:H28]
ATGAACTATGAGCTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTAAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATATCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGACTGGATGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT169851 WP_001270286.1 NZ_CP060065:c2504103-2503687 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT169851 NZ_CP076704:c4204691-4204449 [Escherichia coli O8:H28]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTTACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• PDB: 6HPB