Detailed information of TA system TA169750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 273787..274420
• Replicon: chromosome
• Accession: NZ_CP060047
• Organism: Vibrio vulnificus strain 2497-87

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: H6S61_RS01520
• Protein ID: WP_000843587.1
• Coordinates: 274088..274420 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H6S61_RS01515
• Protein ID: WP_000071008.1
• Coordinates: 273787..274101 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H6S61_RS01465	269177..269320	-	144	WP_185892158.1	DUF3265 domain-containing protein	-
H6S61_RS01470	269323..270237	-	915	WP_103155489.1	alpha/beta hydrolase	-
H6S61_RS23020	270341..270430	-	90	WP_079379794.1	DUF3265 domain-containing protein	-
H6S61_RS01480	270457..270816	-	360	WP_185891264.1	hypothetical protein	-
H6S61_RS01485	270825..270953	-	129	WP_081111502.1	DUF3265 domain-containing protein	-
H6S61_RS01490	270954..271469	-	516	WP_061778617.1	GNAT family N-acetyltransferase	-
H6S61_RS01495	271616..272449	-	834	WP_202988204.1	hypothetical protein	-
H6S61_RS23025	272421..272615	-	195	WP_202988205.1	hypothetical protein	-
H6S61_RS01500	272641..272793	-	153	WP_130314338.1	DUF3265 domain-containing protein	-
H6S61_RS01505	273235..273651	-	417	WP_185891266.1	GNAT family N-acetyltransferase	-
H6S61_RS01510	273680..273790	-	111	WP_080342631.1	DUF3265 domain-containing protein	-
H6S61_RS01515	273787..274101	-	315	WP_000071008.1	DNA-binding transcriptional regulator	Antitoxin
H6S61_RS01520	274088..274420	-	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H6S61_RS01525	274459..274599	-	141	WP_185892161.1	DUF3265 domain-containing protein	-
H6S61_RS01530	274960..275130	-	171	Protein_306	DUF3265 domain-containing protein	-
H6S61_RS01535	275192..275500	+	309	WP_202988212.1	thiamine-binding protein	-
H6S61_RS01540	275536..275695	-	160	Protein_308	DUF3265 domain-containing protein	-
H6S61_RS01545	276001..276129	-	129	WP_185892166.1	DUF3265 domain-containing protein	-
H6S61_RS01550	276489..276578	-	90	WP_202988213.1	DUF3265 domain-containing protein	-
H6S61_RS01555	276609..276851	-	243	WP_029836340.1	hypothetical protein	-
H6S61_RS01560	276924..277013	-	90	WP_202988214.1	DUF3265 domain-containing protein	-
H6S61_RS01565	277031..277507	-	477	WP_140198750.1	GNAT family N-acetyltransferase	-
H6S61_RS01570	277530..277652	-	123	Protein_314	DUF3265 domain-containing protein	-
H6S61_RS01575	277649..277903	-	255	WP_052132573.1	hypothetical protein	-
H6S61_RS01580	277918..278097	-	180	WP_185891270.1	DUF3265 domain-containing protein	-
H6S61_RS01585	278070..278399	-	330	WP_026050567.1	hypothetical protein	-
H6S61_RS01590	278433..278531	-	99	WP_185891272.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	217740..379394	161654
	-	inside	IScluster/Tn	-	-	278528..288359	9831
	-	inside	Integron	-	-	221640..379394	157754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T169750 WP_000843587.1 NZ_CP060047:c274420-274088 [Vibrio vulnificus]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T169750 NZ_CP076689:361218-362138 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11696.20 Da
• Isoelectric Point: 6.7600

>AT169750 WP_000071008.1 NZ_CP060047:c274101-273787 [Vibrio vulnificus]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT169750 NZ_CP076689:360449-361231 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA